| Activation of mouse sperm phosphatidylinositol-4,5 bisphosphate-phospholipase C by zona pellucida is modulated by tyrosine phosphorylation. | |
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MedLine Citation:
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PMID: 8824918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Many cellular responses to the occupancy of membrane receptors include the hydrolysis of phosphatidylinositol-4,5 bisphosphate (PIP2) by phospholipase C (PLC) and the subsequent generation of inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). In the gamete interaction system, sperm respond to binding to the egg's extracellular matrix, the zona pellucida (zp), by exocytosis of the acrosome in a process known as the acrosome reaction (AR). Under physiological conditions, zp binding stimulates ARs only after sperm have undergone a final maturation phase, known as capacitation. One of the zp glycoproteins, ZP3, serves as the ligand for sperm plasma membrane receptors and as the trigger for this regulated exocytosis. Both phosphoinositide-linked and tyrosine kinase-mediated pathways participate in the signalling cascade triggered by sperm-zp interaction. This paper reports that stimulation with solubilized zp increased PIP2-PLC enzymatic activity from mouse sperm. ZP3 is the zp component responsible for this stimulation. The effect was abolished by tyrphostin, suggesting that zp activation of PLC was mediated by tyrosine phosphorylation and that gamma was the PLC isoform involved. We show the presence and distribution of PLC gamma 1 in mouse sperm. Immunostaining studies indicate that PLC gamma 1 is restricted to the sperm head. Sperm capacitation induced translocation of PLC gamma 1 from the soluble to the particulate fraction. These data suggest that PLC gamma 1 constitutes a component in the cascade that couples sperm binding to the egg's extracellular matrix with acrosomal exocytosis, a regulated secretory response upon which fertilization depends absolutely. |
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Authors:
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C N Tomes; C R McMaster; P M Saling |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular reproduction and development Volume: 43 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-11-21 Completed Date: 1996-11-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 196-204 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina 27710, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Enzyme Activation Female Male Mice Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases / metabolism* Phosphorylation Signal Transduction / physiology* Sperm Capacitation / physiology* Sperm-Ovum Interactions / physiology* Spermatozoa / physiology* Tyrosine / metabolism Zona Pellucida / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HD18201/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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55520-40-6/Tyrosine; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.11/Phosphoinositide Phospholipase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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