Document Detail

Activation of mouse ribosomal RNA genes at the 2-cell stage.
MedLine Citation:
PMID:  903155     Owner:  NLM     Status:  MEDLINE    
The Ag-AS method, developed by Goodpasture and Bloom (1975) strains transcriptionally active nucleolus organizer regions (NORs) on the chromosomes and in the interphase nuclei. Metaphases and interphase nuclei of early mouse embryos (unfertilized eggs, pronucleus stages, 2-, 4-, 8-cell stages, and morulae) were subjected to silver-staining. First staining of a single chromosome bearing an NOR was observed at the 2-cell stage. At the 4-cell stage 4--6 chromosomes, and at the 8-cell stage invariably all the 6 chromosomes known to bear NORs, respond positively to silver-staining. These results indicate that during mouse embryogenesis ribosomal RNA genes start to function at the 2-cell stage. The polar body does not respond to silver-staining, which supports the view that the polar body genome remains inactive.
W Engel; M T Zenzes; M Schmid
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  38     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-11-25     Completed Date:  1977-11-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  57-63     Citation Subset:  IM    
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MeSH Terms
Mice / embryology*
RNA, Ribosomal*
Time Factors
Zygote / physiology
Reg. No./Substance:
0/RNA, Ribosomal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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