Document Detail


Activation of mitogen-activated protein kinases (ERK/JNK) and AP-1 transcription factor in rat carotid arteries after balloon injury.
MedLine Citation:
PMID:  9409259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Smooth muscle cell proliferation is a key event in neointimal formation after balloon angioplasty. The molecular signals that mediate this process have yet to be identified. Mitogen-activated protein (MAP) kinases are thought to play a pivotal role in transmitting transmembrane signals required for cell proliferation in vitro. The present studies were designed to investigate whether the signal transduction pathways of MAP kinases were involved in the development of restenosis in the injured arteries. Rat carotid arteries were isolated at various time points after balloon injury, and activities of MAP kinases, including extracellular signal-regulated kinases (ERK), and stress activated protein kinases (SAPK)/c-Jun N-terminal protein kinases (JNK), were determined in protein extracts of the vasculature using protein kinase assay and Western blot analysis. After balloon angioplasty, ERK2 and JNK1 activities in the vessel wall increased rapidly, reached a high level in 5 minutes and maintained for 1 hour. A sustained increase in ERK2 kinase activity was observed over the next 7 days in the arterial wall and 14 days in neointima after injury. In contrast, opposite and uninjured arteries did not show significant changes in these kinase activities. Concomitantly, Western blot analysis confirmed that the ERK2 kinase in the injured vessels was indeed activated or phosphorylated, showing a slowly migrating species of a 42-kDa protein containing phosphorylated tyrosine. Kinase activation is followed by an increase in c-fos and c-jun gene expression and enhanced activator protein 1 (AP-1) DNA-binding activity. Thus, balloon injury rapidly activates the MAP kinases in rat carotid arteries. These kinase activations may be crucial in mediating smooth muscle cell proliferation in response to vascular angioplasty.
Authors:
Y Hu; L Cheng; B W Hochleitner; Q Xu
Related Documents :
12413699 - Lcptp-map kinase interaction: permanent partners or transient associates?
21244889 - Angiotensin ii signal transduction pathways in the cardiovascular system.
8621539 - Erk3 is a constitutively nuclear protein kinase.
22745309 - Fat facets deubiquitylation of medea/smad4 modulates interpretation of a dpp morphogen ...
8632909 - Differential phosphorylations of spi-b and spi-1 transcription factors.
22307599 - Differential regulation of the activity of deleted in liver cancer 1 (dlc1) by tensins ...
8831499 - The fumagillin analogue tnp-470 inhibits dna synthesis of vascular smooth muscle cells ...
8474449 - Cell cycle regulation of the yeast cdc7 protein kinase by association with the dbf4 pro...
25372749 - Endocytosis and the src family of non-receptor tyrosine kinases.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  17     ISSN:  1079-5642     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-22     Completed Date:  1998-01-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2808-16     Citation Subset:  IM    
Affiliation:
Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Angioplasty, Balloon / adverse effects*
Animals
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
Carotid Arteries / metabolism*
Carotid Artery Injuries
DNA / metabolism
Enzyme Activation
Gene Expression Regulation*
Genes, fos
Genes, jun
JNK Mitogen-Activated Protein Kinases
Male
Mitogen-Activated Protein Kinase 1 / metabolism*
Mitogen-Activated Protein Kinases*
Phosphorylation
Protein Binding
Protein Processing, Post-Translational
Rats
Rats, Wistar
Signal Transduction / physiology*
Stress, Mechanical
Transcription Factor AP-1 / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Transcription Factor AP-1; 9007-49-2/DNA; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Long-term probucol treatment reverses the severity of myocardial injury in watanabe heritable hyperl...
Next Document:  Genetic factors precipitating type III hyperlipoproteinemia in hypolipidemic transgenic mice express...