Document Detail

Activation of mitogen-activated protein kinases, activator protein-1, and nuclear factor-kappaB during acute rejection after heterotopic heart transplantation in rats.
MedLine Citation:
PMID:  16986359     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Activation of mitogen-activated protein kinases (MAPKs), including c-Jun NH2-terminal kinases (JNKs), extracellular signal-regulated kinases (ERKs), and p38MAPK during acute cardiac rejection is not clear. This study aimed to determine whether MAPKs and transcriptional factors such as activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) were involved in acute rejection after cardiac transplantation. METHODS: Hearts from Lewis (LEW) rats (group C) or DA rats (group R) were transplanted into the abdomen of recipients (LEW). Grafts were collected at the 1st, 3rd, or 5th postoperative day (POD). ERKs were measured by Western blot analysis, and JNKs were measured by in-gel kinase assay. AP-1 and NF-kappaB DNA binding activities were determined using an electrophoretic mobility shift assay. We assessed functions of donor hearts using echocardiography. RESULTS: Heart rates and myocardial contraction significantly decreased at POD 5 in group R. Phosphorylated p42ERK and p44ERK in the left ventricular free wall (FW) and septal wall (SW) of group R significantly increased at POD 5 compared to those of group C at POD 1. Activities of p46JNK and p55JNK in the FW and SW of group R also significantly increased at POD 5. AP-1 DNA binding activities in the FW and SW of group R significantly increased at POD 5, and NF-kappaB DNA binding activities of group R significantly increased at PODs 3 and 5. CONCLUSIONS: We conclude that ERK, JNK, AP-1, and NF-kappaB are activated during acute rejection. The MAPK pathways may play an important role in acute cardiac rejection.
Hidekazu Hirai; Toshihiko Shibata; Takanobu Aoyama; Minoru Yoshiyama; Takashi Omura; Shigefumi Suehiro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Osaka city medical journal     Volume:  52     ISSN:  0030-6096     ISO Abbreviation:  Osaka City Med J     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-09-21     Completed Date:  2006-10-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376413     Medline TA:  Osaka City Med J     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  9-19     Citation Subset:  IM    
Department of Cardiovascular Surgery, Osaka City University, Graduate School of Medicine, Japan.
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MeSH Terms
Extracellular Signal-Regulated MAP Kinases / physiology*
Graft Rejection / etiology*,  physiopathology
Heart Rate / physiology
Heart Transplantation / adverse effects*,  physiology
JNK Mitogen-Activated Protein Kinases / physiology
NF-kappa B / physiology*
Rats, Inbred Lew
Rats, Inbred Strains
Transcription Factor AP-1 / physiology*
Transplantation, Heterotopic
Reg. No./Substance:
0/NF-kappa B; 0/Transcription Factor AP-1; EC Signal-Regulated MAP Kinases; EC Mitogen-Activated Protein Kinases

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