| Activation-induced cytidine deaminase splicing patterns in chronic lymphocytic leukemia. | |
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MedLine Citation:
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PMID: 20117026 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activation-induced cytidine deaminase (AID) is critically implicated in somatic hypermutation (SHM) and class switch recombination (CSR). AID is expressed as a native transcript and as several splice variants, with as yet undefined roles. Chronic lymphocytic leukemia (CLL) leukemic B cells have also been shown to express AID transcripts, especially in cases with unmutated immunoglobulin (IG) genes. Therefore, AID expression in CLL might potentially be relevant to the disease. The available data on AID-mRNA splicing patterns in CLL are limited and conflicting. Here, we investigated AID-mRNA isoform expression in a series of 195 CLL patients and explored associations with IG gene mutational status and surface immunoglobulin (sIg) isotype expression. Full-length AID transcripts and two splice variants were detected in 110/91/95 cases, respectively. Co-expression of all three AID-mRNA isoforms was significantly more frequent (p<0.001) in cases with unmutated IGHV genes. No significant differences were identified between sIgG vs. sIgMD cases regarding the frequency of AID-mRNA expression. However, expression of at least one AID-mRNA isoform predominated among mutated IgG vs. mutated IgMD cases (p=0.05). These results attest to the biological heterogeneity of CLL and also indicate that AID splice variants may inhibit SHM in CLL cells of the unmutated subtype. |
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Authors:
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Foteini Marantidou; Antonis Dagklis; Evangelia Stalika; Penelope Korkolopoulou; Panagiota Korkolopoulou; Alina Saetta; Achilles Anagnostopoulos; Nikolaos Laoutaris; Kostas Stamatopoulos; Chrysoula Belessi; Zacharias Scouras; Efstratios Patsouris |
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Publication Detail:
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Type: Journal Article Date: 2010-02-08 |
Journal Detail:
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Title: Blood cells, molecules & diseases Volume: 44 ISSN: 1096-0961 ISO Abbreviation: Blood Cells Mol. Dis. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-29 Completed Date: 2010-07-30 Revised Date: 2010-08-12 |
Medline Journal Info:
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Nlm Unique ID: 9509932 Medline TA: Blood Cells Mol Dis Country: United States |
Other Details:
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Languages: eng Pagination: 262-7 Citation Subset: IM |
Affiliation:
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1st Department of Pathology, Laikon General Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece; Hematology Department, Nikea General Hospital, Piraeus, Greece. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Alternative Splicing* B-Lymphocytes / metabolism, pathology Cytidine Deaminase / genetics*, physiology Female Gene Rearrangement, B-Lymphocyte / genetics* Humans Immunoglobulin Class Switching / genetics* Immunoglobulin Heavy Chains / genetics Leukemia, Lymphocytic, Chronic, B-Cell / genetics* Male Middle Aged Neoplasm Proteins / genetics*, physiology Protein Isoforms / genetics, physiology RNA, Messenger / biosynthesis, genetics RNA, Neoplasm / biosynthesis, genetics Reverse Transcriptase Polymerase Chain Reaction Somatic Hypermutation, Immunoglobulin / genetics* VDJ Exons / genetics |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin Heavy Chains; 0/Neoplasm Proteins; 0/Protein Isoforms; 0/RNA, Messenger; 0/RNA, Neoplasm; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
| Comments/Corrections | |
Erratum In:
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Blood Cells Mol Dis. 2010 Aug 15;45(2):180 Note: Korkolopoulou, Panagiota [corrected to Korkolopoulou, Penelope] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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