Document Detail

Activation of human synovial cells by cholera enterotoxin: correlation of morphological responses with adenylate cyclase activities, and the reversing effects of hyaluronidase.
MedLine Citation:
PMID:  6219857     Owner:  NLM     Status:  MEDLINE    
Previously described morphological changes in human synovial cell cultures due to cholera enterotoxin (CT) were studied in relation to activation of adenylate cyclase. A single pulse of CT at nanomolar concentration or less induced at least two-fold activation of adenylate cyclase, which persisted for 7 days or more. The enzyme hyaluronidase was found to cause a rapid reversal of the morphological effects of CT. There was also a reduction in adenylate cyclase activity but only with hyaluronidase concentrations greater than those required to produce maximum reversal of the CT-induced morphological changes. Removal of hyaluronidase was followed by reappearance of the CT-associated morphological effects and a slower reactivation of adenylate cyclase. The mechanism by which hyaluronidase produces the observed changes in synovial cells is not known, but might be related to the dispersal of hyaluronic acid gels bound to the surface of these cells.
B J Clarris; E Baxter; V P Michelangeli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Connective tissue research     Volume:  10     ISSN:  0300-8207     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  1982  
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  173-86     Citation Subset:  IM    
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MeSH Terms
Adenylate Cyclase / analysis*
Cholera Toxin / pharmacology*
Dose-Response Relationship, Drug
Enzyme Activation
Hyaluronic Acid / physiology
Hyaluronoglucosaminidase / pharmacology*
Synovial Membrane / cytology*,  drug effects
Trypsin / pharmacology
Reg. No./Substance:
9004-61-9/Hyaluronic Acid; 9012-63-9/Cholera Toxin; EC; EC; EC Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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