Document Detail


Activation of human neutrophils by mycobacterial phenolic glycolipids.
MedLine Citation:
PMID:  10540187     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interaction between mycobacterial phenolic glycolipids (PGLs) and phagocytes was studied. Human neutrophils were allowed to interact with each of four purified mycobacterial PGLs and the neutrophil production of reactive oxygen metabolites was followed kinetically by luminol-/isoluminol-amplified chemiluminescence. The PGLs from Mycobacterium tuberculosis and Mycobacterium kansasii, respectively, were shown to stimulate the production of oxygen metabolites, while PGLs from Mycobacterium marinum and Mycobacterium bovis BCG, respectively, were unable to induce an oxidative response. Periodate treatment of the M. tuberculosis PGL decreased the production of oxygen radicals, showing the importance of the PGL carbohydrate moiety for the interaction. The activation, however, could not be inhibited by rhamnose or fucose, indicating a complex interaction which probably involves more than one saccharide unit. This is in line with the fact that the activating PGLs from M. tuberculosis and M. kansasii contain tri- and tetrasaccharides, respectively, while the nonactivating PGLs from M. marinum and M. bovis BCG each contain a monosaccharide. The complement receptor 3 (CR3) has earlier been shown to be of importance for the phagocyte binding of mycobacteria, but did not appear to be involved in the activation of neutrophils by PGLs. The subcellular localization of the reactive oxygen metabolites formed was related to the way in which the glycolipids were presented to the cells.
Authors:
J Fäldt; C Dahlgren; A Karlsson; A M Ahmed; D E Minnikin; M Ridell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  118     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-22     Completed Date:  1999-11-22     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  253-60     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology and Immunology, Göteborg University, Göteborg, Sweden. jenny.faldt@microbio.gu.se
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / immunology*
Carbohydrate Metabolism
Carbohydrates / physiology
Complement Inactivator Proteins / pharmacology
Cytoplasmic Granules / metabolism
Cytoskeleton / physiology
Enzyme Induction / immunology
Glycolipids / immunology*
Humans
Macrophage-1 Antigen / metabolism
Monosaccharides / pharmacology
Mycobacterium bovis / chemistry,  immunology
Mycobacterium kansasii / chemistry,  immunology
Mycobacterium marinum / chemistry,  immunology
Mycobacterium tuberculosis / chemistry,  immunology
NADPH Oxidase / biosynthesis
Neutrophil Activation*
Neutrophils / enzymology
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Carbohydrates; 0/Complement Inactivator Proteins; 0/Glycolipids; 0/Macrophage-1 Antigen; 0/Monosaccharides; 0/phenolic glycolipid I, Mycobacterium leprae; EC 1.6.3.1/NADPH Oxidase
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