| Activation of human immunodeficiency virus by herpes simplex virus. | |
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MedLine Citation:
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PMID: 1354237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heterologous viruses have been examined for their ability to accelerate the course of infection with the human immunodeficiency virus (HIV) type 1. In this study, ACH-2 cells persistently infected with HIV-1 exhibited augmented HIV-1 replication as a result of superinfection with herpes simplex virus (HSV) type 1. Using HSV-1 mutants with deletions in the genes encoding immediate-early proteins ICP0, ICP4, and ICP27, it was found that ICP0 and ICP27, but not ICP4, were essential for up-regulation of HIV replication. Northern blot analysis showed that this activation of HIV was characterized by an initial rise in the level of the small, subgenomic (2.0 and 4.3 kb) mRNA species, followed by an increase in the level of unspliced genomic (9.2 kb) mRNA. Such a shift in transcriptional phase recapitulates the early-to-late transition seen in single-step growth curves of acute HIV-1 infection. Thus, HSV can activate HIV-1 from latency in ACH-2 cells, this activation of HIV is independent of productive HSV replication since the delta ICP4 deletion mutant is replication-incompetent, and this activation is evident as an increase in the steady-state levels of HIV transcripts. |
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Authors:
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M P Golden; S Kim; S M Hammer; E A Ladd; P A Schaffer; N DeLuca; M A Albrecht |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 166 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1992-09-17 Completed Date: 1992-09-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 494-9 Citation Subset: AIM; IM; X |
Affiliation:
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Division of Infectious Diseases, New England Deaconess Hospital, Boston, MA 02215. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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CD4-Positive T-Lymphocytes
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cytology,
microbiology HIV-1 / genetics, growth & development* Humans Kinetics Mutation RNA, Viral / biosynthesis Simplexvirus / genetics, physiology* Tumor Cells, Cultured Virus Activation* Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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AI-01015/AI/NIAID NIH HHS; AI-30897/AI/NIAID NIH HHS; HL-43510/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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