Document Detail

Activation of guanylate cyclase by arachidonic acid in mammary gland homogenates from mice.
MedLine Citation:
PMID:  30119     Owner:  NLM     Status:  MEDLINE    
Arachidonic acid stimulated guanylate cyclase activity about two fold in homogenates of mammary glands obtained from midpregnant mice; effects of arachidonic acid were observed during incubation periods between 5 and 20 minutes. Stimulatory effects of arachidonic acid on guanylate cyclase activity were observed when 10 to 100 microgram arachidonic acid was added to the reaction mixtures (150 microliter). When 250 microgram or more arachidonic acid was added to the reaction mixtures, the activity of guanylate cyclase was inhibited. Other fatty acids including linoleic acid, linolenic acid and oleic acid also stimulated guanylate cyclase activity but neither arachidic acid nor stearic acid had an effect. The arachidonic acid stimulation of guanylate cyclase activity was abolished by incubation with indomethacin and aspirin, thus suggesting the arachidonic acid effect may be carried out via the prostaglandins. A variety of prostaglandins, however, at several concentrations did not stimulate guanylate cyclase activity when added to the reaction mixtures. The failure of the prostaglandins to have an effect may be due to several reasons which are discussed.
J A Rillema
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Prostaglandins     Volume:  15     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1978 May 
Date Detail:
Created Date:  1978-12-20     Completed Date:  1978-12-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  857-65     Citation Subset:  IM    
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MeSH Terms
Arachidonic Acids / pharmacology*
Enzyme Activation / drug effects
Fatty Acids / pharmacology
Guanylate Cyclase / metabolism*
Indomethacin / pharmacology
Mammary Glands, Animal / drug effects,  enzymology*
Reg. No./Substance:
0/Arachidonic Acids; 0/Fatty Acids; 53-86-1/Indomethacin; EC Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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