Document Detail


Activation of glycolysis by zinc is diminished in hepatocytes from metallothionein-null mice.
MedLine Citation:
PMID:  11051599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of hepatic metallothionein (MT) and zinc (Zn) on glycolysis was investigated in primary cultures of mouse hepatocytes prepared from MT-normal (+/+) and MT-null (-/-) mice. In MT +/+ mice, a close relationship was observed between the Zn concentration in the incubation medium (10-150 microM), increased MT levels in the cells, and increased glycolysis (accumulation of lactate + pyruvate) over 24 h, with significant effects seen at physiological levels of Zn (10-25 microM). Hepatocytes from MT -/- mice had significantly lower basal rates of glycolysis and demonstrated increased glycolysis only at Zn concentrations of 50 microM or greater. The lactate:pyruvate ratio was higher in the MT +/+ hepatocytes. The oxidation of endogenous fatty acid (accumulation of the ketone bodies, 3-hydroxybutyrate and acetoacetate) was initially greater in the MT +/+ hepatocytes, although only MT -/- hepatocytes showed increased ketone body production in response to Zn. The 3-hydroxybutyrate:acetoacetate ratio was higher in the MT +/+ hepatocytes and increased with increasing Zn concentrations. Intracellular Zn accumulation was 60% greater in the MT +/+ hepatocytes, with approximately 80% of the extra Zn associated with MT. The results implicate MT-associated Zn rather than increased intracellular Zn per se in the regulation of hepatic carbohydrate metabolism.
Authors:
A M Rofe; J C Philcox; P Coyle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological trace element research     Volume:  75     ISSN:  0163-4984     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2000  
Date Detail:
Created Date:  2000-11-16     Completed Date:  2000-11-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  87-97     Citation Subset:  IM    
Affiliation:
Division of Clinical Biochemistry, Institute of Medical and Veterinary Science, Adelaide, Australia SA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Metabolism
Cells, Cultured
Glycolysis / drug effects*
Hepatocytes / drug effects,  metabolism*
Metallothionein / genetics,  metabolism,  physiology*
Mice
Mice, Inbred C57BL
Mice, Knockout
Zinc / pharmacology*
Chemical
Reg. No./Substance:
7440-66-6/Zinc; 9038-94-2/Metallothionein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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