Document Detail

Activation of a cyclic nucleotide phosphodiesterase 4 (PDE4) from rat thymocytes by phosphatidic acid.
MedLine Citation:
PMID:  9023016     Owner:  NLM     Status:  MEDLINE    
The cytosolic cyclic nucleotide phosphodiesterase (PDE) activity from rat thymocytes was resolved into five peaks by HPLC. Only two forms of the cAMP-specific PDE4 were found to be sensitive to physiologically relevant phosphatidic acid (PA) concentrations. PA activated the PDE4-peak 3 form, the fatty acid composition and unsaturation degree determining the efficiency of PA. The PDE4-peak 2 form was inhibited only by PA with saturated fatty acyl groups. PDE4 activation was specific of anionic phospholipids, a free phosphate group in the phospholipid molecule being required for maximum activation. These results suggest that PA may contribute to the lowering of cAMP level required in the early steps of a lympho-proliferative response, thus regulating immune functions through PDE4 activation.
A Savany; C Abriat; G Némoz; M Lagarde; A F Prigent
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular signalling     Volume:  8     ISSN:  0898-6568     ISO Abbreviation:  Cell. Signal.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-04-17     Completed Date:  1997-04-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  511-6     Citation Subset:  IM    
INSERM U.352, Laboratoire de Biochimie et Pharmacologie, INSA-Lyon, Villeurbanne, France.
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MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases*
Cells, Cultured
Cyclic Nucleotide Phosphodiesterases, Type 4
Cytosol / enzymology
Enzyme Activation
Lipids / pharmacology
Phosphatidic Acids / chemistry,  pharmacology*
Phosphoric Diester Hydrolases / metabolism*
Rats, Sprague-Dawley
Structure-Activity Relationship
Thymus Gland / cytology,  enzymology*
Reg. No./Substance:
0/Lipids; 0/Phosphatidic Acids; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC',5'-Cyclic-AMP Phosphodiesterases; EC Nucleotide Phosphodiesterases, Type 4

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