| Activation of caspase-3-like enzymes in non-apoptotic T cells. | |
| | |
MedLine Citation:
|
PMID: 9541584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The activation of the caspase family of cysteine proteases is a key step in the implementation of apoptotic cell death leading to further downstream effects such as DNA fragmentation. In cultured tumor cells, caspase activity appears only when cells are undergoing apoptosis. Here we show that human and murine T lymphocytes acquire high intracellular activities of cell death-specific caspases upon activation by mitogens and IL-2 without evidence that apoptosis is proceeding. The highest activity is seen when cells are mitogen activated for 3 days. On a per cell basis, caspase activity in activated T cells is much higher than in tumor cells induced to undergo apoptosis. In the presence of exogenously added IL-2 cells stay alive and maintain a high level of caspase activity while IL-2 withdrawal results in cell death and decline of caspase activity. Caspase activity can also be measured in extracts from spleen and lymph nodes from mice injected with superantigen. While in tumor cell lines caspase activity correlates with cleavage of poly(ADP)-ribose polymerase (PARP) and DNA fragmentation, in activated T cells cleavage products of cellular PARP can be detected whereas DNA fragmenting activity appears only upon IL-2 withdrawal which coincides with cell death. These data show that caspase activation in intact cells does not necessarily lead to cell death and argue for a checkpoint in the apoptotic pathway downstream of caspases. Furthermore, they provide a molecular correlate for the high susceptibility of activated T cells for apoptosis. |
| | |
Authors:
|
S Wilhelm; H Wagner; G Häcker |
Related Documents
:
|
12931034 - Characteristics of apoptotic cell death induced by coxsackievirus b in permissive vero ... 16848514 - Induction of cell death in caco-2 human colon carcinoma cells by ellagic acid rich frac... 15122584 - Linear relationship between wnt activity levels and apoptosis in colorectal carcinoma c... 12690294 - Cell cycle block and apoptosis induction in a human melanoma cell line following treatm... 4000974 - Hormonal regulation of transcription of rdna: glucocorticoid effects upon initiation an... 19829014 - Transfection with pax6 gene of mouse embryonic stem cells and subsequent cell cloning i... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of immunology Volume: 28 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 1998 Mar |
Date Detail:
|
Created Date: 1998-04-23 Completed Date: 1998-04-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 891-900 Citation Subset: IM |
Affiliation:
|
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Annexin A5 / metabolism Apoptosis* Caspase 3 Caspases* Cells, Cultured Cysteine Endopeptidases / metabolism* DNA Fragmentation Enterotoxins / pharmacology Enzyme Activation Female Humans Interleukin-2 / pharmacology Lymphocyte Activation* Mice Mice, Inbred BALB C Mice, Inbred C57BL Phosphatidylserines / metabolism Poly(ADP-ribose) Polymerases Proteins / metabolism T-Lymphocytes / enzymology* Up-Regulation |
| Chemical | |
Reg. No./Substance:
|
0/Annexin A5; 0/Enterotoxins; 0/Interleukin-2; 0/Phosphatidylserines; 0/Proteins; 39424-53-8/enterotoxin B, staphylococcal; EC 2.4.2.30/PARP1 protein, human; EC 2.4.2.30/Parp1 protein, mouse; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases; EC 3.4.22.-/Cysteine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Human 4-1BB regulates CD28 co-stimulation to promote Th1 cell responses.
Next Document: CD40 ligation and IL-4 use different mechanisms of transcriptional activation of the human lymphotox...