| Activation of the canonical Wnt/beta-catenin pathway enhances monocyte adhesion to endothelial cells. | |
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MedLine Citation:
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PMID: 16815294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monocyte adhesion to vascular endothelium has been reported to be one of the early processes in the development of atherosclerosis. In an attempt to develop strategies to prevent or delay atherosclerosis progression, we analyzed effects of the Wnt/beta-catenin signaling pathway on monocyte adhesion to various human endothelial cells. Adhesion of fluorescein-labeled monocytes to various human endothelial cells was analyzed under a fluorescent microscope. Unlike sodium chloride, lithium chloride enhanced monocyte adhesion to endothelial cells in a dose-dependent manner. We further demonstrated that inhibitors for glycogen synthase kinase (GSK)-3beta or proteosome enhanced monocyte-endothelial cell adhesion. Results of semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) indicated that activation of Wnt/beta-catenin pathway did not change expression levels of mRNA for adhesion molecules. In conclusion, the canonical Wnt/beta-catenin pathway enhanced monocyte-endothelial cell adhesion without changing expression levels of adhesion molecules. |
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Authors:
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Dong Kun Lee; R Nathan Grantham; Aaron L Trachte; John D Mannion; Colleen L Wilson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-22 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 347 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-11 Completed Date: 2006-09-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 109-16 Citation Subset: IM |
Affiliation:
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Cardiovascular Care Center, Comanche County Memorial Hospital, Lawton, OK 73502, USA. leek@memorialhealthsource.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion
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drug effects,
physiology* Cells, Cultured Dose-Response Relationship, Drug Endothelial Cells / cytology, drug effects, physiology* Humans Lithium / administration & dosage Monocytes / cytology, drug effects, physiology* Signal Transduction / drug effects, physiology* Wnt Proteins / metabolism* beta Catenin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/CTNNB1 protein, human; 0/Wnt Proteins; 0/beta Catenin; 7439-93-2/Lithium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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