Document Detail


Activation of calcium (Ca)-dependent protein kinase C in aged mesenteric lymph node T and B cells.
MedLine Citation:
PMID:  8458634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the effects of aging on the activities and translocation of Ca-dependent protein kinase C (PKC) in resting mesenteric lymph node (MLN) T and B cells during the activation process induced by T and B cell mitogens and B cell-stimulatory interleukins, including IL-4, IL-5 and IL-6. The activation process in senescent, resting (high density (HD)) MLN T cells is impaired, when these cells are stimulated with T cell mitogen, Con A. The defect in activation is associated with a reduction in both the new production of inositol-1,4,5-triphosphate (IP3) (an indicator for the production of intracellular free Ca) and the induction of Ca-dependent PKC. In contrast, the activation of the aged B cells with LPS plus/minus interleukins (IL-4, IL-5 and IL-6) is not impaired, being at least associated with a Ca-independent pathway of PKC activation. The elevated IP3 content and total (cytosol plus membrane) PKC activity in both resting T and B cells from aged MLN along with the greater difference in T cells than in B cells suggest that the in vivo Go cell cycle status of these cells may differ from that of the young, involving more in T cells. Finally, the MLN and splenic T cell Ca-dependent and B cell Ca-independent PKC activation do not differ between both age groups.
Authors:
H Kawanishi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Immunology letters     Volume:  35     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-04-23     Completed Date:  1993-04-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-32     Citation Subset:  IM    
Affiliation:
Gut Mucosal Molecular Immunity Laboratory, Department of Medicine, UMDNJ Robert Wood Johnson Medical School, New Brunswick.
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MeSH Terms
Descriptor/Qualifier:
Aging / immunology,  metabolism
Animals
B-Lymphocytes / enzymology*,  immunology
Calcium / metabolism
Enzyme Activation
Inositol 1,4,5-Trisphosphate / metabolism
Interleukins / pharmacology
Lymph Nodes / cytology
Lymphocyte Activation
Male
Mice
Mice, Inbred BALB C
Protein Kinase C / metabolism*
Second Messenger Systems
T-Lymphocytes / enzymology*,  immunology
Grant Support
ID/Acronym/Agency:
7R0IAG07603/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Interleukins; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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