| Activation of calcium (Ca)-dependent protein kinase C in aged mesenteric lymph node T and B cells. | |
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MedLine Citation:
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PMID: 8458634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the effects of aging on the activities and translocation of Ca-dependent protein kinase C (PKC) in resting mesenteric lymph node (MLN) T and B cells during the activation process induced by T and B cell mitogens and B cell-stimulatory interleukins, including IL-4, IL-5 and IL-6. The activation process in senescent, resting (high density (HD)) MLN T cells is impaired, when these cells are stimulated with T cell mitogen, Con A. The defect in activation is associated with a reduction in both the new production of inositol-1,4,5-triphosphate (IP3) (an indicator for the production of intracellular free Ca) and the induction of Ca-dependent PKC. In contrast, the activation of the aged B cells with LPS plus/minus interleukins (IL-4, IL-5 and IL-6) is not impaired, being at least associated with a Ca-independent pathway of PKC activation. The elevated IP3 content and total (cytosol plus membrane) PKC activity in both resting T and B cells from aged MLN along with the greater difference in T cells than in B cells suggest that the in vivo Go cell cycle status of these cells may differ from that of the young, involving more in T cells. Finally, the MLN and splenic T cell Ca-dependent and B cell Ca-independent PKC activation do not differ between both age groups. |
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Authors:
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H Kawanishi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Immunology letters Volume: 35 ISSN: 0165-2478 ISO Abbreviation: Immunol. Lett. Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-04-23 Completed Date: 1993-04-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7910006 Medline TA: Immunol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 25-32 Citation Subset: IM |
Affiliation:
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Gut Mucosal Molecular Immunity Laboratory, Department of Medicine, UMDNJ Robert Wood Johnson Medical School, New Brunswick. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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immunology,
metabolism Animals B-Lymphocytes / enzymology*, immunology Calcium / metabolism Enzyme Activation Inositol 1,4,5-Trisphosphate / metabolism Interleukins / pharmacology Lymph Nodes / cytology Lymphocyte Activation Male Mice Mice, Inbred BALB C Protein Kinase C / metabolism* Second Messenger Systems T-Lymphocytes / enzymology*, immunology |
| Grant Support | |
ID/Acronym/Agency:
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7R0IAG07603/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukins; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate; EC 2.7.11.13/Protein Kinase C |
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