Document Detail

Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells.
MedLine Citation:
PMID:  20524209     Owner:  NLM     Status:  MEDLINE    
Lysozyme is a major component of airway epithelial secretions, acts as cationic anti-microbial protein for innate immunity. Although lysozyme plays an important role in airway defense and is a key component of airway secretions under inflammatory conditions, little is understood about the regulation of its expression and the associated signaling pathway. We wanted to examine whether Phorbol 12-myristate 13-acetate (PMA), one of PKC activators, treatment of the airway epithelial cell line NCI-H292 increases lysozyme gene expression. In this study, we sought to determine which signal molecules are involved in PMA-induced lysozyme gene expression. We found that PKC and mitogen-activating protein/ERK2 kinase are essential for PMA-induced lysozyme expression and also mediate the PMA-induced activation of c-Myb protein. We identified a proximal region of the lysozyme promoter essential for promoter activity containing c-Myb transcription factor binding site. Additionally, by site-directed promoter mutagenesis, we identified that c-Myb preferred the CAA motif of the -85/-73 region of the lysozyme promoter. Finally, we showed that overexpression of c-Myb without PMA treatment increased the lysozyme promoter activity and protein expression. From these results, we conclude that PMA induces overexpression of lysozyme via ERK1/2 MAP kinase-c-Myb signaling pathways in NCI-H292 cells.
Uk Yeol Moon; Jung Ho Bae; Chang-Hoon Kim; Hyun Jik Kim; Ju Wan Kang; Joo-Heon Yoon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  111     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  476-87     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Korea.
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MeSH Terms
Anti-Infective Agents
Cell Line
Epithelial Cells / metabolism*
Extracellular Signal-Regulated MAP Kinases / metabolism
Gene Expression / drug effects*
Muramidase / drug effects,  genetics*
Proto-Oncogene Proteins c-myb / metabolism*
Respiratory System / cytology*
Signal Transduction / drug effects
Tetradecanoylphorbol Acetate / pharmacology*
Transcription Factors
Reg. No./Substance:
0/Anti-Infective Agents; 0/Proto-Oncogene Proteins c-myb; 0/Transcription Factors; 16561-29-8/Tetradecanoylphorbol Acetate; EC Signal-Regulated MAP Kinases; EC

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