Document Detail


Activation of c-Jun N-terminal kinase 1 (JNK-1) after amino acid deficiency in HeLa cells.
MedLine Citation:
PMID:  11384840     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Long-term amino acid starvation represents a form of metabolic stress which stimulates gene expression. Here we report that depriving HeLa cells for any one of a series of amino acids activates c-Jun N-terminal kinase-1 (JNK-1). In contrast, the other mitogen-activated protein kinases (MAPKs) ERK-1 and, to a lesser extent, p38 activities decreased under such conditions. In methionine- or leucine-deprived cells, JNK-1 activation occurred after 4 or 6 h, respectively, and reached a steady maximum of 5- to 7-fold over control cells afterwards. This activation was dependent on the amino acid concentration and it could be reversed by resupplying the complete medium. Limitation for all amino acids also augmented JNK-1 activity, whereas increased amino acid concentrations had an opposite effect. The free radical scavenging thiol antioxidant N-acetylcysteine (NAC) alleviated partially JNK-1 activation in amino acid-deprived cells. The data indicate that activation of JNK-1 by long-term amino acid deprivation may be mediated in part by oxidative stress.
Authors:
C Aubel; S Dehez; H Chabanon; C Seva; M Ferrara; P Brachet
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular signalling     Volume:  13     ISSN:  0898-6568     ISO Abbreviation:  Cell. Signal.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-31     Completed Date:  2001-07-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  England    
Other Details:
Languages:  eng     Pagination:  417-23     Citation Subset:  IM    
Affiliation:
INRA U.R. 238 Nutrition Cellulaire et Moléculaire, 63122 Saint Gener Champanelle, France.
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology
Amino Acids / deficiency*
Enzyme Activation
Free Radical Scavengers / pharmacology
Hela Cells
Humans
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases / metabolism*
Oxidative Stress
Precipitin Tests
Substrate Specificity
Time Factors
p38 Mitogen-Activated Protein Kinases
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Free Radical Scavengers; 616-91-1/Acetylcysteine; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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