Document Detail

Activation of beta1 integrins induces cell-cell adhesion.
MedLine Citation:
PMID:  11161706     Owner:  NLM     Status:  MEDLINE    
Integrins are highly regulated receptors that can function in both cell-substrate and cell-cell adhesion. We have found that the activating anti-beta1 mAb, 12G10, can specifically and rapidly induce both cell-substrate and cell-cell adhesion of HT-1080 human fibrosarcoma and other cell types. Binding of mAb 12G10 induced clustering of cell-surface integrins, and the preferential localization of beta1 integrins expressing the 12G10 epitope at cell-cell adhesion sites. Fab fragments of mAb 12G10 induced HT-1080 cell-cell adhesion as effectively as did intact antibodies, suggesting that integrin clustering was not due to direct antibody crosslinking. Latrunculin B, an inhibitor of F-actin polymerization, inhibited cell-cell adhesion but not the clustering of integrins. Results from a novel, two-color cell-cell adhesion assay suggested that nonactivated cells can bind to activated cells and that integrin activation-induced HT-1080 cell-cell adhesion minimally requires the interaction of activated alpha2beta1 with nonactivated alpha3beta1. These findings suggest that HT-1080 cell-cell adhesion induced by integrin activation require a signaling process involving integrin clustering and the subsequent organization of the cytoskeleton. Integrin activation could therefore play a key role in cell-cell adhesion.
J D Whittard; S K Akiyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  263     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  65-76     Citation Subset:  IM    
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
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MeSH Terms
Actins / metabolism
Antibodies, Monoclonal / immunology,  metabolism
Antigens, CD29 / immunology,  metabolism*
Bicyclo Compounds, Heterocyclic / pharmacology
Cadherins / metabolism
Cations, Divalent / metabolism
Cell Adhesion / physiology*
Collagen / metabolism
Dose-Response Relationship, Immunologic
Fibronectins / metabolism
Laminin / metabolism
Microscopy, Confocal
Models, Biological
Signal Transduction
Thiazoles / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Actins; 0/Antibodies, Monoclonal; 0/Antigens, CD29; 0/Bicyclo Compounds, Heterocyclic; 0/Cadherins; 0/Cations, Divalent; 0/Epitopes; 0/Fibronectins; 0/Laminin; 0/Thiazoles; 0/Thiazolidines; 76343-94-7/latrunculin B; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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