| Activation of beta1 integrins induces cell-cell adhesion. | |
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MedLine Citation:
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PMID: 11161706 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Integrins are highly regulated receptors that can function in both cell-substrate and cell-cell adhesion. We have found that the activating anti-beta1 mAb, 12G10, can specifically and rapidly induce both cell-substrate and cell-cell adhesion of HT-1080 human fibrosarcoma and other cell types. Binding of mAb 12G10 induced clustering of cell-surface integrins, and the preferential localization of beta1 integrins expressing the 12G10 epitope at cell-cell adhesion sites. Fab fragments of mAb 12G10 induced HT-1080 cell-cell adhesion as effectively as did intact antibodies, suggesting that integrin clustering was not due to direct antibody crosslinking. Latrunculin B, an inhibitor of F-actin polymerization, inhibited cell-cell adhesion but not the clustering of integrins. Results from a novel, two-color cell-cell adhesion assay suggested that nonactivated cells can bind to activated cells and that integrin activation-induced HT-1080 cell-cell adhesion minimally requires the interaction of activated alpha2beta1 with nonactivated alpha3beta1. These findings suggest that HT-1080 cell-cell adhesion induced by integrin activation require a signaling process involving integrin clustering and the subsequent organization of the cytoskeleton. Integrin activation could therefore play a key role in cell-cell adhesion. |
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Authors:
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J D Whittard; S K Akiyama |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental cell research Volume: 263 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-02-22 Completed Date: 2001-03-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 65-76 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Antibodies, Monoclonal / immunology, metabolism Antigens, CD29 / immunology, metabolism* Bicyclo Compounds, Heterocyclic / pharmacology Cadherins / metabolism Cations, Divalent / metabolism Cell Adhesion / physiology* Collagen / metabolism Dose-Response Relationship, Immunologic Epitopes Fibronectins / metabolism Fibrosarcoma Humans Hybridomas Immunohistochemistry Laminin / metabolism Microscopy, Confocal Models, Biological Signal Transduction Thiazoles / pharmacology Thiazolidines Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Antibodies, Monoclonal; 0/Antigens, CD29; 0/Bicyclo Compounds, Heterocyclic; 0/Cadherins; 0/Cations, Divalent; 0/Epitopes; 0/Fibronectins; 0/Laminin; 0/Thiazoles; 0/Thiazolidines; 76343-94-7/latrunculin B; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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