| Activation of atypical protein kinase Czeta toward TC10 is regulated by high-fat diet and aerobic exercise in skeletal muscle. | |
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MedLine Citation:
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PMID: 18702941 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We determined whether sustained aerobic exercise reverses high-fat diet-induced impairments in the c-Cbl associated protein (CAP)/Casitas b-lineage lymphoma (c-Cbl) signaling cascade in rodent skeletal muscle. Sprague-Dawley rats were placed into either control (n = 16) or high-fat-fed (n = 32) diet groups for 4 weeks. During a subsequent 4-week experimental period, 16 high-fat-fed rats remained sedentary, 16 high-fat-fed rats completed 4 weeks of exercise training, and control animals were sedentary and remained on the control diet. After the intervention period, animals were subjected to hind limb perfusions in the presence (n = 8 per group) or absence (n = 8 per group) of insulin. In the plasma membrane fractions, neither high-fat feeding nor exercise training altered adaptor protein with PH and SH2 domains, (APS), c-Cbl, or TC10 protein concentrations. In contrast, CAP protein concentration and insulin-stimulated plasma membrane c-Cbl tyrosine phosphorylation were reduced by high-fat feeding; but exercise training reversed these impairments. Of note was that insulin-stimulated atypical protein kinase Czeta kinase activity toward TC10 was reduced by high-fat feeding but normalized by exercise training. We conclude that sustained (4 weeks) exercise training can reverse high-fat diet-induced impairments on the CAP/c-Cbl pathway in high-fat-fed rodent skeletal muscle. We also provide the first evidence that the CAP/c-Cbl insulin signaling cascade in skeletal muscle may directly interact with components of the classic (phosphoinositide 3-kinase dependent) insulin signaling cascade. |
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Authors:
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Misato Saito; S J Lessard; Donato A Rivas; Donald W Reeder; John A Hawley; Ben B Yaspelkis |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 57 ISSN: 1532-8600 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-15 Completed Date: 2008-09-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: United States |
Other Details:
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Languages: eng Pagination: 1173-80 Citation Subset: IM |
Affiliation:
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Exercise Biochemistry Laboratory, Department of Kinesiology, California State University Northridge, CA 91330-8287, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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metabolism Animals Cell Membrane / metabolism Cytoskeletal Proteins / blood, metabolism* Dietary Fats / administration & dosage*, pharmacology Enzyme Activation / drug effects, physiology Insulin / metabolism, pharmacology Isoenzymes / metabolism Male Membrane Proteins / metabolism Muscle, Skeletal / metabolism, physiology* Osmolar Concentration Phosphorylation / drug effects Physical Conditioning, Animal / physiology* Protein Kinase C / metabolism* Proto-Oncogene Proteins c-cbl / blood, metabolism* Rats Rats, Sprague-Dawley Signal Transduction / drug effects Threonine / metabolism Tyrosine / metabolism rho GTP-Binding Proteins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK-57625/DK/NIDDK NIH HHS; R15 DK057625-02/DK/NIDDK NIH HHS; R15 DK057625-03/DK/NIDDK NIH HHS; S06 GM048680-110020/GM/NIGMS NIH HHS; T34 GM008395-10/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Aps protein, rat; 0/Cytoskeletal Proteins; 0/Dietary Fats; 0/Isoenzymes; 0/Membrane Proteins; 0/flotillins; 11061-68-0/Insulin; 55520-40-6/Tyrosine; 72-19-5/Threonine; EC 2.7.1.37/protein kinase C lambda; EC 2.7.11.1/protein kinase C zeta; EC 2.7.11.13/Protein Kinase C; EC 3.6.1.-/Rhoq protein, rat; EC 3.6.5.2/rho GTP-Binding Proteins; EC 6.3.2.-/Proto-Oncogene Proteins c-cbl |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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