| Activation of apoptotic caspase-3 and nitric oxide synthase-2 in buccal mucosa with chronic alcohol ingestion. | |
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MedLine Citation:
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PMID: 9762419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis, the process of programmed cell death, involves activation of caspase proteases cascade that remains under the regulatory control of nitric oxide. In this study, we investigated the activity of a key apoptotic protease, caspase-3, and the expression of nitric oxide synthase-2 (NOS-2) associated with buccal epithelial cells apoptosis induced by chronic ethanol diet. The assays revealed that a 7.9-fold enhancement in buccal epithelial cells apoptosis, observed in the alcohol diet group, was accompanied by a 37.6-fold increase in caspase-3 activity and a 10.1-fold increase in NOS-2. Furthermore, the expression of NOS-2 showed a positive correlation (r = 0.92) with the extent of changes induced in caspase-3 activity. These results implicate caspase-3 in the process of alcohol-induced epithelial cells apoptosis, and point towards participation of NOS-2 in the amplification of the cell death signaling cascade. |
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Authors:
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B L Slomiany; J Piotrowski; E Piotrowski; A Slomiany |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry and molecular biology international Volume: 45 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-12-14 Completed Date: 1998-12-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 1199-209 Citation Subset: IM |
Affiliation:
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Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcoholism
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enzymology*,
pathology* Animals Apoptosis* / drug effects Caspase 3 Caspases / metabolism* Enzyme Induction Ethanol / toxicity Mouth Mucosa / enzymology, pathology* Nitric Oxide Synthase / metabolism* Nitric Oxide Synthase Type II Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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64-17-5/Ethanol; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, rat; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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