| Activation of antibody Fc function by antigen-induced conformational changes. | |
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MedLine Citation:
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PMID: 1061094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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IgM antibody directed against the pheny-beta-lactoside hapten was examined for its capacity to fix complement in the presence of the hapten, monohapten-substituted antigen, and multihapten-substituted antigen. Hapten was found to have no effect; monovalent antigen induced an excellent response which could be inhibited by hapten; and multivalent antigen also induced an excellent response which was related to the number of determinants added and not to the formation of antigen-antibody aggregates. The difference between the activities of hapten and monovalent antigen was reflected in their affinities for the IgM antibody. The monovalent antigen had a lower Ka, indicating that energy from binding was used to activate the Fc complement binding sites. These data show that the expression of IgM Fc function depends on a change in Fc conformation produced by the binding of antigen at the distant Fab combining sites. |
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Authors:
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J C Brown; M E Koshland |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 72 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1975 Dec |
Date Detail:
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Created Date: 1976-03-30 Completed Date: 1976-03-30 Revised Date: 2010-09-03 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5111-5 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Binding Sites, Antibody Complement Fixation Tests Complement System Proteins Haptens Humans Immunoglobulin Fc Fragments* Immunoglobulin M* Kinetics Molecular Weight Protein Binding Protein Conformation Ribonucleases |
| Chemical | |
Reg. No./Substance:
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0/Binding Sites, Antibody; 0/Haptens; 0/Immunoglobulin Fc Fragments; 0/Immunoglobulin M; 9007-36-7/Complement System Proteins; EC 3.1.-/Ribonucleases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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