Document Detail

Activation of the alternative complement pathway and production of factor H by skeletal myotubes.
MedLine Citation:
PMID:  8505407     Owner:  NLM     Status:  MEDLINE    
Skeletal muscle myotubes from neonatal rats were used to study the interaction of skeletal muscle with complement. Serum from guinea pig, rabbit, and human, in the absence of muscle-specific antibody, caused creatine phosphokinase release, which required activation of the terminal complement cascade. Cleavage of serum C3 and Factor B in the presence of myotubes was dependent on Mg2+, but not Ca2+, and C3 cleavage occurred only in the presence of Factor B. Rat myotubes caused significant consumption of C8 and C9 in rat serum, which also required Mg2+, but not Ca2+. All of these findings are typical of a tissue capable of activating the alternative pathway. In addition, the C2 myotube cell line was shown to produce Factor H, an inhibitory protein of the alternative pathway, as demonstrated by Factor H mRNA expression and immunoprecipitation of the protein.
T J Lang; M L Shin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  44     ISSN:  0165-5728     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-02     Completed Date:  1993-07-02     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
Department of Pathology, University of Maryland School of Medicine, Baltimore.
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MeSH Terms
Complement C3 / metabolism
Complement Factor H / biosynthesis*,  genetics
Complement Membrane Attack Complex / metabolism
Complement Pathway, Alternative*
Guinea Pigs
Muscles / immunology*,  metabolism
RNA, Messenger / analysis
Reg. No./Substance:
0/Complement C3; 0/Complement Membrane Attack Complex; 0/RNA, Messenger; 0/complement factor H, human; 80295-65-4/Complement Factor H

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