Document Detail


Activation of SRF-regulated chromosomal templates by Rho-family GTPases requires a signal that also induces H4 hyperacetylation.
MedLine Citation:
PMID:  9491889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Constitutively active forms of the small GTPases RhoA (RhoA.V14) and Cdc42 (Cdc42.V12) induce expression of extrachromosomal SRF reporter genes in microinjection experiments, but only Cdc42.V12 can efficiently activate a chromosomal template. Both SAPK/JNK-dependent or -independent signals can cooperate with RhoA.V14 to activate chromosomal SRF reporters, and it is SAPK/JNK activation by Cdc42.V12 that allows it to activate chromosomal templates. Cooperating signals can be bypassed by deacetylase inhibitors. Three findings show that histone H4 hyperacetylation is one target for cooperating signals, although it alone is not sufficient: (1) Cdc42.V12, but not RhoA.V14, induces H4 hyperacetylation; (2) cooperating signals use the same SAPK/JNK-dependent or -independent pathways to induce H4 hyperacetylation; (3) growth factor and stress stimuli induce substantial H4 hyperacetylation, detectable in reporter gene chromatin. These data establish a link between signal-regulated acetylation events and gene transcription.
Authors:
A S Alberts; O Geneste; R Treisman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  92     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-19     Completed Date:  1998-03-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  475-87     Citation Subset:  IM    
Affiliation:
Transcription Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Acetylation
Animals
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Cell Cycle Proteins / metabolism*
Chromatin / metabolism
Chromosomes / chemistry
DNA-Binding Proteins / genetics*
GTP Phosphohydrolases / metabolism
GTP-Binding Proteins / metabolism*
Gene Expression Regulation, Enzymologic
Genes, Reporter
Genes, fos / genetics*
Histones / metabolism*
Mice
Mitogen-Activated Protein Kinases*
Nuclear Proteins / genetics*
Serum Response Factor
Signal Transduction / physiology
Transcription Factors / genetics
cdc42 GTP-Binding Protein
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
p38 Mitogen-Activated Protein Kinases
rhoA GTP-Binding Protein
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromatin; 0/DNA-Binding Proteins; 0/Histones; 0/Nuclear Proteins; 0/Serum Response Factor; 0/Transcription Factors; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 3.6.1.-/GTP Phosphohydrolases; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.5.2/cdc42 GTP-Binding Protein; EC 3.6.5.2/cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; EC 3.6.5.2/rhoA GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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