Document Detail


Activation of NK1.1+ T cells in vitro and their possible role in age-associated changes in inducible IL-4 production.
MedLine Citation:
PMID:  9259768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interleukin (IL)-12 or IL-4 produced early in an immune response directs the differentiation of naive antigen-activated CD4+ T cells down a Th1 or Th2 pathway. The NK1.1+ subset of T cells promptly produces IL-4 following activation in vivo. We demonstrate here that NK1.1+ T cells can be directly induced to produce IL-4 in vitro when activated under serum-free culture conditions. Platelet-derived growth factor in cell culture medium was inhibitory to the production of IL-4 by NK1.1+ T cells in vitro. Lymphocytes obtained from secondary lymphoid organs of aged mice produced greater quantities of IL-4 following stimulation than lymphocytes from mature adult animals. Aged mice expressed elevated percentages of NK1.1+ T cells in their secondary lymphoid organs and peripheral blood. While this cell type was responsible for the total early IL-4 produced by lymphocytes from mature adult mice, both NK1.1+ and memory phenotype (CD44high, CD45RBlow, NK1.1-) T cells from aged donors produced IL-4 following polyclonal T cell activation.
Authors:
M E Poynter; H H Mu; X P Chen; R A Daynes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular immunology     Volume:  179     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-09-08     Completed Date:  1997-09-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  22-9     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics
Aging / immunology*
Animals
Antigens / immunology*
Antigens, CD3 / immunology
Antigens, CD44 / immunology
Antigens, CD45 / immunology
Antigens, Ly
Antigens, Surface
CD4-Positive T-Lymphocytes / immunology*
Cells, Cultured
Female
Interleukin-2 / biosynthesis
Interleukin-4 / biosynthesis*,  genetics
Lectins, C-Type
Lymphocyte Activation*
Mice
Mice, Inbred C57BL
NK Cell Lectin-Like Receptor Subfamily B
Platelet-Derived Growth Factor / pharmacology
Proteins / immunology*
RNA, Messenger
Receptors, Antigen, T-Cell, alpha-beta / immunology
Spleen / cytology,  immunology
Grant Support
ID/Acronym/Agency:
5T32 HD07491/HD/NICHD NIH HHS; AG11475/AG/NIA NIH HHS; CA25917/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Antigens; 0/Antigens, CD3; 0/Antigens, CD44; 0/Antigens, Ly; 0/Antigens, Surface; 0/Interleukin-2; 0/Klrb1c protein, mouse; 0/Lectins, C-Type; 0/NK Cell Lectin-Like Receptor Subfamily B; 0/Platelet-Derived Growth Factor; 0/Proteins; 0/RNA, Messenger; 0/Receptors, Antigen, T-Cell, alpha-beta; 0/platelet-derived growth factor BB; 207137-56-2/Interleukin-4; EC 3.1.3.48/Antigens, CD45

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