Document Detail


Activation of the Mammalian cells by using light-sensitive ion channels.
MedLine Citation:
PMID:  22573444     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
With advances in molecular biology and gene cloning techniques, it is now possible to selectively stimulate living cells of interest by using an external light source. This is done by transfecting the cells of interest with a plasmid carrying the channelrhodopsin (ChR2) gene. By stimulating these transfected cells with laser, the light-sensitive ion channels ChR2 are opened, followed by an influx of cation resulting in cell activation. This combination of optical and genetic technique is known in the literature as optogenetics. It is particularly useful in the functional studies of excitable cells, such as neurons, muscle and endocrine cells, to mimic the stimulation from action potentials to trigger the release neurotransmitters and hormones. Here, we describe the methods needed to make selected mammalian cells (PC12) respond to light excitation.
Authors:
Mandy Siu Yu Lung; Paul Pilowsky; Ewa M Goldys
Related Documents :
3620464 - Regulation of mitochondrial hexokinase in cultured ht 29 human cancer cells. an ultrast...
16036614 - How to clean the dirtiest place in the cell: cationic antioxidants as intramitochondria...
659264 - Inhibition of lactate dehydrogenase in cultured sirc cells by cigarette smoke.
20532184 - Effectiveness of novel borane-phosphine complexes in inhibiting cell death depends on t...
23013534 - Oxamflatin improves developmental competence of porcine somatic cell nuclear transfer e...
11017794 - Mutations selected in rotavirus enterotoxin nsp4 depend on the context of its expression.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  875     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  241-51     Citation Subset:  IM    
Affiliation:
Australian School of Advanced Medicine, Macquarie University, North Ryde, NSW, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Initial stages of angiosperm greening monitored by low-temperature fluorescence spectra and fluoresc...
Next Document:  Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytome...