Document Detail

Activation of the Mammalian cells by using light-sensitive ion channels.
MedLine Citation:
PMID:  22573444     Owner:  NLM     Status:  In-Data-Review    
With advances in molecular biology and gene cloning techniques, it is now possible to selectively stimulate living cells of interest by using an external light source. This is done by transfecting the cells of interest with a plasmid carrying the channelrhodopsin (ChR2) gene. By stimulating these transfected cells with laser, the light-sensitive ion channels ChR2 are opened, followed by an influx of cation resulting in cell activation. This combination of optical and genetic technique is known in the literature as optogenetics. It is particularly useful in the functional studies of excitable cells, such as neurons, muscle and endocrine cells, to mimic the stimulation from action potentials to trigger the release neurotransmitters and hormones. Here, we describe the methods needed to make selected mammalian cells (PC12) respond to light excitation.
Mandy Siu Yu Lung; Paul Pilowsky; Ewa M Goldys
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  875     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  241-51     Citation Subset:  IM    
Australian School of Advanced Medicine, Macquarie University, North Ryde, NSW, Australia.
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