Document Detail


Activation of DNA damage response signaling in lung adenocarcinoma A549 cells following oxygen beam irradiation.
MedLine Citation:
PMID:  21609781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxygen beams are high linear energy transfer (LET) radiation characterized by higher relative biological effectiveness than low LET radiation. The aim of the current study was to determine the signaling differences between γ- and oxygen ion-irradiation. Activation of various signaling molecules was looked in A549 lung adenocarcinoma cells irradiated with 2Gy oxygen, 2Gy or 6Gy γ-radiation. Oxygen beam was found to be three times more cytotoxic than γ-radiation. By 4h there was efficient repair of DNA in A549 cells exposed to 2Gy or 6Gy gamma radiation but not in cells exposed to 2Gy oxygen beam as determined by γ-H2AX counting. Number of ATM foci was found to be significantly higher in cells exposed to 2Gy oxygen beam. Percentage of cells showing ATR foci were more with gamma however number of foci per cell were more in case of oxygen beam. Oxygen beam irradiated cells showed phosphorylation of Chk1, Chk2 and p53. Many apoptotic nuclei were seen by DAPI staining in cells exposed to oxygen beam. The noteworthy finding of this study is the activation of the sensor proteins, ATM and ATR by oxygen irradiation and the significant activation of Chk1, Chk2 and p53 only in the oxygen beam irradiated cells.
Authors:
Somnath Ghosh; Himanshi Narang; Asiti Sarma; Harminder Kaur; Malini Krishna
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-14
Journal Detail:
Title:  Mutation research     Volume:  723     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-01     Completed Date:  2011-09-15     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  190-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Radiation Biology and Health Science Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. somnath@barc.gov.in
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics*
Cell Cycle Proteins / metabolism
Cell Line, Tumor
DNA Damage / radiation effects*
DNA Repair
DNA-Binding Proteins
Gamma Rays
Histones / metabolism
Humans
Linear Energy Transfer*
Lung Neoplasms / genetics*
Oxygen*
Protein-Serine-Threonine Kinases / metabolism
Signal Transduction / genetics
Tumor Suppressor Proteins / metabolism
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/H2AFX protein, human; 0/Histones; 0/Tumor Suppressor Proteins; 7782-44-7/Oxygen; EC 2.7.1.-/ATR protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/ataxia telangiectasia mutated protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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