Document Detail


Activation of a Cl-dependent K flux by cAMP in pig red cells.
MedLine Citation:
PMID:  2539726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Activation of a Cl-dependent K flux by adenosine 3',5'-cyclic monophosphate (cAMP) was characterized in pig red cells, a cell type that lacks both the Ca-activated K channel and the Na-K-Cl cotransport pathway. As in other red cells, both Cl-dependent K efflux and K influx are stimulated on cell swelling. Although pig red cells fail to respond to beta-adrenergic stimuli, it is possible to raise the intracellular cAMP content by preincubating cells in the presence of 1 mM cAMP. The Cl-dependent K flux was compared in cells having a basal cAMP content of approximately 0.29 nmol/g hemoglobin vs. cAMP-loaded cells having approximately 8.4 nmol cAMP/g hemoglobin. Loading with cAMP stimulated both Cl-dependent K efflux and influx of hypotonically swollen cells. In maximally swollen cells whose volume was increased by approximately 17%, the Cl-dependent Rb influx occurs with a maximum velocity (Vmax) of 17.9 +/- 3.2 mumol.g hemoglobin (Hb)-1.h-1 and Km for Rb of 22.9 +/- 4.1 mM. In cAMP-loaded cells, both Vmax and Km were increased to 59.8 +/- 8.5 mumol.g Hb-1.h-1 and 63.1 +/- 8.8 mM, respectively. The Cl-dependent Rb influx is much larger in young cells than in old cells. However, both cell types respond to cAMP activation. Whereas cAMP and its analogues, 8-bromoadenosine 3',5'-cyclic monophosphate and dibutyryl adenosine 3',5'-cyclic monophosphate are stimulatory, AMP and guanosine 3',5'-cyclic monophosphate (cGMP) are not. These findings suggest that, like other ion transport systems, the Cl-dependent K flux of pig red cells is endowed with the capacity to respond to cAMP.
Authors:
H D Kim; S Sergeant; L R Forte; D H Sohn; J H Im
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  256     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-18     Completed Date:  1989-05-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C772-8     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, School of Medicine, University of Missouri-Columbia 65212.
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / analogs & derivatives,  pharmacology
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Animals
Biological Transport / drug effects
Bucladesine / pharmacology
Bumetanide / pharmacology
Calcium / blood
Carrier Proteins / blood
Chlorides / pharmacology*
Cyclic AMP / blood,  pharmacology*
Erythrocytes / drug effects,  metabolism*
Ethylmaleimide / pharmacology
Furosemide / pharmacology
Kinetics
Ouabain / pharmacology
Potassium / blood*
Rubidium / blood
Sodium-Potassium-Chloride Symporters
Swine
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
ID/Acronym/Agency:
DK-33456/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Chlorides; 0/Sodium-Potassium-Chloride Symporters; 128-53-0/Ethylmaleimide; 16561-29-8/Tetradecanoylphorbol Acetate; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 27816-59-7/4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 28395-03-1/Bumetanide; 362-74-3/Bucladesine; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 54-31-9/Furosemide; 60-92-4/Cyclic AMP; 630-60-4/Ouabain; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7440-70-2/Calcium

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