| Activation of B cell apoptotic pathways in the course of Francisella tularensis infection. | |
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MedLine Citation:
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PMID: 20600796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Francisella tularensis is a facultative intracellular, gram-negative bacterium that induces apoptosis in macrophages and B cells. Here we show apoptotic pathways that are activated in the Ramos human B cell line in the course of F. tularensis infection. Live bacteria F. tularensis FSC200 activate caspases 8, 9 and 3, as well as Bid; release cytochrome c and apoptosis-inducing factor from mitochondria; and induce depolarization of mitochondrial membrane potential in the Ramos cell line, thus leading these cells to apoptosis. Unlike live bacteria, killed F. tularensis FSC200 bacteria activated only caspase 3, and did not cause apoptosis of Ramos cells as measured by annexin V. Killed bacteria also caused accumulation of anti-apoptotic protein Bclx(L) in mitochondrial membranes. Thus, live F. tularensis activates both caspase pathways (receptor-mediated and intrinsic) as well as caspase-independent mitochondrial death. |
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Authors:
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Lucie Zivna; Zuzana Krocova; Anetta Härtlova; Klara Kubelkova; Jitka Zakova; Emil Rudolf; Roman Hrstka; Ales Macela; Jiri Stulik |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-23 |
Journal Detail:
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Title: Microbial pathogenesis Volume: 49 ISSN: 1096-1208 ISO Abbreviation: Microb. Pathog. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-08-10 Completed Date: 2010-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8606191 Medline TA: Microb Pathog Country: England |
Other Details:
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Languages: eng Pagination: 226-36 Citation Subset: IM |
Copyright Information:
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Copyright 2010. Published by Elsevier India Pvt Ltd. |
Affiliation:
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Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* B-Lymphocytes / microbiology* BH3 Interacting Domain Death Agonist Protein / biosynthesis Caspase 3 / biosynthesis Caspase 8 / biosynthesis Caspase 9 / biosynthesis Cell Line Cytochromes c / metabolism Francisella tularensis / pathogenicity* Humans Membrane Potential, Mitochondrial Mitochondria / enzymology, physiology |
| Chemical | |
Reg. No./Substance:
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0/BH3 Interacting Domain Death Agonist Protein; 0/BID protein, human; 9007-43-6/Cytochromes c; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9 |
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