Document Detail

Activated gammadelta T cells promote the activation of uveitogenic T cells and exacerbate EAU development.
MedLine Citation:
PMID:  21296823     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine how the activation of γδ T cells affects the generation of uveitogenic αβ T cells and the development of experimental autoimmune uveitis (EAU).
METHODS: γδ T cells were isolated from B6 mice immunized with the uveitogenic peptide IRBP(1-20) and αβ T cells from immunized TCR-δ(-/-) mice. Resting γδ T cells were prepared by culture of separated γδ T cells in cytokine-free medium for 3 to 5 days, when they showed downregulation of CD69 expression. Activated γδ T cells were prepared by incubating resting γδ T cells with anti-γδ TCR (GL3) for 2 days. Responder αβ T cells were cocultured with immunizing antigen and antigen-presenting cells. The numbers of antigen-specific T cells expressing IL-17 or IFN-γ were determined by intracellular staining followed by FACS analysis after stimulation, with or without the addition of purified γδ T cells. The cytokines in the culture medium were measured by ELISA.
RESULTS: Highly enriched γδ T cells exert widely different effects on autoreactive αβ T cells in EAU, depending on the activation status of the γδ T cells. Whereas nonactivated γδ T cells had little effect on the activation of interphotoreceptor retinoid-binding protein-specific αβ T cells in vitro and in vivo, activated γδ T cells promoted the generation of uveitogenic T cells and exacerbated the development of EAU.
CONCLUSIONS: The functional ability of γδ T cells is greatly influenced by their activation status. Activated γδ T cells exacerbate EAU through increased activation of uveitogenic T cells.
Hong Nian; Hui Shao; Rebecca L O'Brien; Willi K Born; Henry J Kaplan; Deming Sun
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-07-29
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  52     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-08-01     Completed Date:  2011-09-30     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5920-7     Citation Subset:  IM    
Doheny Eye Institute, Department of Ophthalmology, University of Southern California, Los Angeles, California 90033, USA.
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MeSH Terms
Autoimmune Diseases / immunology*
Eye Proteins / immunology,  pharmacology
Interleukin-17 / immunology
Lymphocyte Activation / immunology*
Mice, Inbred C57BL
Mice, Mutant Strains
Peptide Fragments / immunology,  pharmacology
Receptors, Antigen, T-Cell, gamma-delta / genetics*,  immunology*
Retinol-Binding Proteins / immunology,  pharmacology
Severity of Illness Index
Specific Pathogen-Free Organisms
Uveitis / immunology*
Grant Support
EY003040/EY/NEI NIH HHS; EY017373/EY/NEI NIH HHS; EY018827/EY/NEI NIH HHS; R01 EY017373-05/EY/NEI NIH HHS; R01 EY018827-01A2/EY/NEI NIH HHS; R01 EY018827-02/EY/NEI NIH HHS; R01 EY018827-03/EY/NEI NIH HHS; R01 EY022403/EY/NEI NIH HHS
Reg. No./Substance:
0/Eye Proteins; 0/Interleukin-17; 0/Peptide Fragments; 0/Receptors, Antigen, T-Cell, gamma-delta; 0/Retinol-Binding Proteins; 0/interstitial retinol-binding protein

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