Document Detail

Activated calcineurin confers high tolerance to ion stress and alters the budding pattern and cell morphology of yeast cells.
MedLine Citation:
PMID:  8798496     Owner:  NLM     Status:  MEDLINE    
The PP2B protein phosphatase, also known as calcineurin, is a regulator of ion homeostasis in yeast cells. We have investigated the physiological consequences of constitutive expression of a recombinant form of calcineurin in which the Ca2+/calmodulin-binding and autoinhibitory domains of the catalytic subunit were deleted. The concomitant expression of the regulatory subunit along with the truncated catalytic subunit resulted in high tolerance to toxic levels of Na+ and Li+. This activated form of calcineurin substituted for the Na+ stress signal to promote the expression of the ENA1 gene, encoding a P-ATPase pump, and to induce the transition of the K+ uptake system to the high affinity mode that restricts influx of Na+ and Li+. In addition, the transcriptional responsiveness of ENA1 to Na+ stress was enhanced. These results demonstrate that calcineurin has a pivotal role in a signaling cascade activated by ion stress in yeast. Moreover, we found that changes in the level of calcineurin activity affected budding pattern and cell morphology. Cells expressing the truncated calcineurin were elongated and budded in an unipolar pattern, whereas calcineurin-deficient mutants budded randomly. These results suggest that calcineurin may also act in the establishment of cell polarity.
I Mendoza; F J Quintero; R A Bressan; P M Hasegawa; J M Pardo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-11-19     Completed Date:  1996-11-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23061-7     Citation Subset:  IM    
Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, P. O. Box 1052, Sevilla 41080, Spain.
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MeSH Terms
Biological Transport
Calmodulin-Binding Proteins / genetics,  metabolism*
Cations, Monovalent / metabolism,  toxicity*
Cell Division / physiology
Drug Resistance, Microbial / physiology*
Homeostasis / physiology*
Lithium / metabolism,  toxicity
Morphogenesis / physiology
Phosphoprotein Phosphatases / genetics,  metabolism*
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / physiology*
Sodium / metabolism,  toxicity
Structure-Activity Relationship
Reg. No./Substance:
0/Calmodulin-Binding Proteins; 0/Cations, Monovalent; 0/Recombinant Proteins; 7439-93-2/Lithium; 7440-23-5/Sodium; EC; EC Phosphatases

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