| Activated α(2) macroglobulin induces matrix metalloproteinase 9 expression by low-density lipoprotein receptor-related protein 1 through MAPK-ERK1/2 and NF-κB activation in macrophage-derived cell lines. | |
| | |
MedLine Citation:
|
PMID: 20568116 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Macrophages under certain stimuli induce matrix metalloproteinase 9 (MMP-9) expression and protein secretion through the activation of MAPK-ERK and NF-κB signaling pathways. Previously, we demonstrated that activated α(2)-macroglulin (α(2)M*) through the interaction with its receptor low-density lipoprotein receptor-related protein 1 (LRP1) induces macrophage proliferation mediated by the activation of MAPK-ERK1/2. In the present work, we examined whether α(2)M*/LRP1interaction could induce the MMP-9 production in J774 and Raw264.7 macrophage-derived cell lines. It was shown that α(2)M* promoted MMP-9 expression and protein secretion by LRP1 in both macrophage-derived cell lines, which was mediated by the activation of MAPK-ERK1/2 and NF-κB. Both intracellular signaling pathways activated by α(2)M* were effectively blocked by calphostin-C, suggesting involvement of PKC. In addition, we demonstrate that α(2)M* produced extracellular calcium influx via LRP1. However, when the intracellular calcium mobilization was inhibited by BAPTA-AM, the α(2)M*-induced MAPK-ER1/2 activation was fully blocked in both macrophage cell lines. Finally, using specific pharmacological inhibitors for PKC, Mek1, and NF-κB, it was shown that the α(2)M*-induced MMP-9 protein secretion was inhibited, indicating that the MMP production promoted by the α(2)M*/LRP1 interaction required the activation of both signaling pathways. These findings may prove useful in the understanding of the macrophage LRP1 role in the vascular wall during atherogenic plaque progression. |
| | |
Authors:
|
Leandro C Cáceres; Gustavo R Bonacci; María C Sánchez; Gustavo A Chiabrando |
Related Documents
:
|
19101986 - Radiation-induced hif-1alpha cell survival pathway is inhibited by soy isoflavones in p... 16905546 - Nqo1 and nqo2 regulation of humoral immunity and autoimmunity. 15155616 - Pneumocystis activates human alveolar macrophage nf-kappab signaling through mannose re... 12925536 - Inositol 1,3,4-trisphosphate 5/6-kinase inhibits tumor necrosis factor-induced apoptosis. 9341746 - Unmethylated cpg motifs protect murine b lymphocytes against fas-mediated apoptosis. 19416216 - Role of relaxin in regulation of fibrosis in the lung. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 111 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-27 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 607-17 Citation Subset: IM |
Copyright Information:
|
© 2010 Wiley-Liss, Inc. |
Affiliation:
|
Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Centro de Investigaciones en Bioquímica Clínica e Inmunología, Ciudad Universitaria 5000, Córdoba, Argentina. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Atherosclerosis Calcium Signaling Cell Line LDL-Receptor Related Protein 1 / physiology* Macrophages / metabolism* Matrix Metalloproteinase 9 / biosynthesis*, genetics, secretion Mice Mitogen-Activated Protein Kinase 3 / metabolism* Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism* Signal Transduction Transcriptional Activation alpha-Macroglobulins / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/LDL-Receptor Related Protein 1; 0/NF-kappa B; 0/alpha-Macroglobulins; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reactive oxygen and nitrogen species during meiotic resumption from diplotene arrest in mammalian oo...
Next Document: Cellular stress induced by resazurin leads to autophagy and cell death via production of reactive ox...