Document Detail


Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (Atrax robustus), in anaesthetized monkeys.
MedLine Citation:
PMID:  2728033     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Robustoxin, a polypeptide consisting of a chain of 42 amino acid residues in a known sequence, has been isolated by cation exchange chromatography from the crude venom of the male funnel-web spider (Atrax robustus). Physiological activity or toxicity in the venom fractions was detected by production of fasciculation in mouse phrenic nerve-hemidiaphragm preparations and by lethality in new-born mice. In the present experiments in Macaca fascicularis monkeys anaesthetized with pentobarbitone, robustoxin (5-30 micrograms/kg infused i.v. over 5 min) produced immediate disturbances in respiration (including dyspnoea and apnoea), blood pressure and heart rate followed by severe hypotension (mean systemic blood pressure less than 50 mmHg) or death due to respiratory and circulatory failure within 196 min. Robustoxin also produced lachrymation, salivation, generalized skeletal muscle fasciculation and a parallel increase in body temperature, and increased firing in skeletal motor and autonomic nerves. These effects closely resembled those produced by i.v. infusions over 5 min of 50 micrograms/kg of crude venom from male A. robustus spiders. Crude venom from female A. robustus spiders (500 micrograms/kg i.v. over 5 min) produced some of the effects elicited by robustoxin and crude venom from male spiders, but to a much less marked extent. It was concluded that robustoxin is responsible for the neurotoxic and lethal effects of human envenomation by male A. robustus spiders.
Authors:
E J Mylecharane; I Spence; D D Sheumack; R Claassens; M E Howden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  27     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1989  
Date Detail:
Created Date:  1989-06-29     Completed Date:  1989-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  481-92     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Sydney, N.S.W. Australia.
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MeSH Terms
Descriptor/Qualifier:
Anesthesia
Animals
Arthropod Venoms / toxicity*
Blood Pressure / drug effects
Crying / physiology
Electromyography
Female
Heart Rate / drug effects
Injections, Intramuscular
Injections, Intravenous
Macaca fascicularis
Male
Salivation / drug effects
Spider Venoms / toxicity*
Chemical
Reg. No./Substance:
0/Arthropod Venoms; 0/Spider Venoms; 91728-12-0/robustoxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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