| Actions of lipoxygenase metabolites in isolated rat lungs. | |
| | |
MedLine Citation:
|
PMID: 6436213 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Leukotrienes constrict smooth muscle and could be important for the regulation of the pulmonary circulation. We examined the production and action of lipoxygenase metabolites in isolated lungs, where we controlled the perfusing fluid used. Arachidonate injected into isolated rat lungs perfused with cell- and protein-free physiological salt solution caused a transient pressor response. Following indomethacin, arachidonate caused a delayed slow pressure rise followed by edema. The lung effluent contracted the guinea pig ileum. High-pressure liquid chromatography (HPLC) analysis of the perfusate demonstrated the presence of leukotrienes (LTC4 and LTD4). Diethylcarbamazine, a leukotriene synthesis inhibitor, prevented the slow pressure rise and edema seen after indomethacin plus arachidonate. In lungs perfused with cell- and protein-free physiological salt solution, LTC4, but not LTD4, caused a transient pressure rise followed by a sustained pressure rise. The sustained rise was abolished by a leukotriene-receptor blocker (FPL 55712) but not by indomethacin. In blood-perfused lungs, LTC4 caused only the transient pressure rise that was not blocked by FPL 55712. In lungs perfused with physiological salt solution containing albumin, LTC4 had no effect. We concluded that 1) perfused nonsensitized rat lungs produced LTC4 and LTD4; 2) LTC4 may be a major pulmonary vasoconstrictor; and 3) albumin binding limits the pressor effect of LTC4. |
| | |
Authors:
|
N F Voelkel; K R Stenmark; J T Reeves; M M Mathias; R C Murphy |
Related Documents
:
|
6208233 - Lung oedema--microscopic detection. 8472073 - Evaluation of human diaphragm function. 933213 - Analysis of the response to therapeutic measures to reduce intracranial pressure in hea... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of applied physiology: respiratory, environmental and exercise physiology Volume: 57 ISSN: 0161-7567 ISO Abbreviation: J Appl Physiol Publication Date: 1984 Sep |
Date Detail:
|
Created Date: 1984-11-30 Completed Date: 1984-11-30 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7801242 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 860-7 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonic Acid Arachidonic Acids / pharmacology Chromatography, High Pressure Liquid Chromones / pharmacology Diethylcarbamazine / pharmacology Hydroxyeicosatetraenoic Acids / pharmacology Indomethacin / pharmacology Lung / drug effects, metabolism* Male Muscle Contraction / drug effects Muscle, Smooth / drug effects, physiology Perfusion Pressure Pulmonary Circulation Rats Rats, Inbred Strains SRS-A / antagonists & inhibitors, metabolism*, pharmacology Serum Albumin, Bovine / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
HL-01047/HL/NHLBI NIH HHS; HL-14985/HL/NHLBI NIH HHS; HL-25785/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Arachidonic Acids; 0/Chromones; 0/Hydroxyeicosatetraenoic Acids; 0/SRS-A; 0/Serum Albumin, Bovine; 40786-08-1/FPL 55712; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin; 90-89-1/Diethylcarbamazine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pulmonary neutrophil kinetics after thrombin-induced intravascular coagulation.
Next Document: Perihepatitis (Fitz-Hugh--Curtis syndrome). A review and case presentation.