Document Detail

Actions of insulin on MCF-7 cells that are synchronized with hydroxyurea.
MedLine Citation:
PMID:  3308577     Owner:  NLM     Status:  MEDLINE    
The role of insulin in stimulating metabolic processes in MCF-7 cells was studied in cells synchronized at the G1:S interphase of the cell cycle using hydroxyurea. Cells released from the hydroxyurea block progressed through one S-phase of the cell cycle when insulin was absent from the medium. When free insulin was present the cells continued through more than one S-phase. Since cells accumulate at G0 in serum- and hormone-free conditions it is apparent that insulin has an essential action in the MCF-7 cells between the G0 and S-phase of the cell cycle. Insulin is also known to stimulate the incorporation of [3H]leucine into a pH 4.6 precipitable phosphoprotein fraction in the MCF-7 cells. Insulin was shown to express this action, even when the cells were maintained at the G1:S interphase with hydroxyurea. Insulin is thus able to effect a differentiative action, i.e. a stimulation of phosphoprotein synthesis, under conditions where insulin's effect on [3H]thymidine incorporation into DNA is prevented.
B E Linebaugh; J A Rillema
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  52     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1987-11-05     Completed Date:  1987-11-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201.
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MeSH Terms
Cell Cycle / drug effects*
Cell Line
DNA / biosynthesis
Hydroxyurea / pharmacology*
Insulin / pharmacology*
Interphase / drug effects
Grant Support
Reg. No./Substance:
11061-68-0/Insulin; 127-07-1/Hydroxyurea; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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