Document Detail

Actions of PACAP and VIP on melanotrope cells of Xenopus laevis.
MedLine Citation:
PMID:  17482316     Owner:  NLM     Status:  MEDLINE    
The neuropeptides, pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) are implicated in the regulation of gene expression and hormone secretion in mammalian melanotrope cells and a mammalian pro-opiomelanocortin (POMC)-producing tumor cell line, but the physiological relevance of this regulation is elusive. The purpose of the present study was to establish if these peptides affect biosynthetic and secretory processes in a well-established physiological model for endocrine cell functioning, the pituitary melanotrope cells of the amphibian Xenopus laevis, which hormonally control the process of skin color adaptation to background illumination. We show that both PACAP and VIP are capable of stimulating the secretory process of the Xenopus melanotrope cell. As the peptides are equipotent, they may exert their actions via a VPAC receptor. Moreover, PACAP stimulated POMC biosynthesis and POMC gene expression. Strong anti-PACAP immunoreactivity was found in the pituitary pars nervosa (PN), suggesting that this neurohemal organ is a source of neurohormonal PACAP action on the melanotropes in the intermediate pituitary. We propose that the PACAP/VIP family of peptides has a physiological function in regulating Xenopus melanotrope cell activity during the process of skin color adaptation.
Adhanet H Kidane; Peter M J M Cruijsen; Maria A Ortiz-Bazan; Hubert Vaudry; Jerome Leprince; Frouwke J Kuijpers-Kwant; Eric W Roubos; Bruce G Jenks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-30
Journal Detail:
Title:  Peptides     Volume:  28     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-10     Completed Date:  2008-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1790-6     Citation Subset:  IM    
Department of Cellular Animal Physiology, Faculty of Science, Integrative Physiology, EURON, Radboud University Nijmegen, 6525 ED Nijmegen, The Netherlands.
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MeSH Terms
Dose-Response Relationship, Drug
Gene Expression / drug effects
Melanotrophs / cytology,  drug effects*,  metabolism
Pituitary Adenylate Cyclase-Activating Polypeptide / pharmacology*
Pituitary Gland / metabolism
Pro-Opiomelanocortin / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Vasoactive Intestinal Peptide / pharmacology*
Xenopus laevis
Reg. No./Substance:
0/Pituitary Adenylate Cyclase-Activating Polypeptide; 37221-79-7/Vasoactive Intestinal Peptide; 66796-54-1/Pro-Opiomelanocortin

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