Document Detail


Action spectrum for expression of the high intensity light-inducible Lhc-like gene Lhl4 in the green alga Chlamydomonas reinhardtii.
MedLine Citation:
PMID:  16418228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lhl4 encodes a distant relative of light-harvesting Chl-a/b proteins in the green alga Chlamydomonas reinhardtii. Lhl4 mRNA markedly accumulated within 30 min after illumination and in proportion to the light intensity up to a fluence rate much higher than that required for photosynthesis. The high intensity light (HL)-induced accumulation of Lhl4 mRNA required continuous illumination, and the mRNA level rapidly decreased when the cells were placed in the dark. HL only slightly stabilized the mRNA, suggesting that the HL-induced expression of the Lhl4 gene is primarily regulated at the level of transcription. Blue light was more effective for inducing Lhl4 gene expression than green or red light, and far-red light had no effect. The action spectrum for Lhl4 gene expression was examined at wavelengths between 325 and 775 nm using the Okazaki Large Spectrograph. The obtained spectrum showed a distinct peak in the blue region (450 nm) and a shoulder in the UV-A region (375 nm). The curve in the spectrum rose steeply in the short wavelength UV region. In addition, we observed two minor peaks in the green (575 nm) and the red (675 nm) regions. The action spectrum suggests that a blue/UV-A light photoreceptor with a flavin-based chromophore participates in the HL response of Lhl4 gene expression. However, the hypersensitivity to near UV-B light suggests the involvement of an unidentified UV light perception system in the expression of the Lhl4 gene.
Authors:
Haruhiko Teramoto; Asako Ishii; Yukihiro Kimura; Koji Hasegawa; Shigeaki Nakazawa; Takanori Nakamura; Sho-ichi Higashi; Masakatsu Watanabe; Taka-aki Ono
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-17
Journal Detail:
Title:  Plant & cell physiology     Volume:  47     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-04-06     Completed Date:  2006-07-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  419-25     Citation Subset:  IM    
Affiliation:
Laboratory for Photo-Biology (1), Photodynamics Research Center, RIKEN, Sendai, Japan. teramoto@riken.or.jp
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MeSH Terms
Descriptor/Qualifier:
Algal Proteins / genetics,  metabolism
Animals
Cells, Cultured
Chlamydomonas reinhardtii / genetics*,  physiology,  radiation effects*
Darkness
Gene Expression Regulation*
Light*
Light-Harvesting Protein Complexes / genetics*,  metabolism*
Protozoan Proteins / genetics,  metabolism
RNA Stability / radiation effects
RNA, Messenger / genetics,  metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Algal Proteins; 0/Light-Harvesting Protein Complexes; 0/Protozoan Proteins; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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