| Action of peptidases in brain synaptic membranes on the NH2-terminus of adrenocorticotropin using ACTH-(1-16)-NH2 as a model substrate. | |
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MedLine Citation:
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PMID: 6299290 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The action of brain peptidases on NH2-terminal sequences of adrenocorticotropin was studied by incubation of ACTH-(1-16)-NH2 under different pH conditions. Profiles of metabolites and time course of product formation were obtained by HPLC analysis of the digests. Fragments of ACTH-(1-16)-NH2 were isolated and characterized by their amino acid composition and NH2-terminal groups. Both at pH 7.4 and pH 8.5 the following fragments were found: ACTH-(3-16)-NH2, ACTH-(4-16)-NH2, ACTH-(5-16)-NH2, and ACTH-(7-16)-NH2. At pH 7.4 the major products were ACTH-(4-16)-NH2 and ACTH-(7-16)-NH2, while the peptide ACTH-(3-16)-NH2 was the main metabolite at pH 8.5. The nature of identified peptides and the time course of their formation demonstrates that aminopeptidase activities predominate in the conversion of the NH2-terminus of adreno-corticotropin and related peptides by brain synaptic membranes. |
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Authors:
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X C Wang; J P Burbach; J C Verhoef |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 111 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-04-15 Completed Date: 1983-04-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 259-65 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenocorticotropic Hormone
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analogs & derivatives*,
metabolism Amino Acids / analysis Animals Brain / enzymology* Chromatography, High Pressure Liquid Male Peptide Fragments / metabolism* Peptide Hydrolases / metabolism* Rats Rats, Inbred Strains Synaptic Membranes / enzymology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Peptide Fragments; 18806-03-6/ACTH amide (1-16); 9002-60-2/Adrenocorticotropic Hormone; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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