Document Detail

Action of luteinizing hormone-releasing hormone: involvement of novel arachidonic acid metabolites.
MedLine Citation:
PMID:  6344087     Owner:  NLM     Status:  MEDLINE    
Anterior pituitary cells were incubated in the presence of luteinizing hormone-releasing hormone and one of three inhibitors of arachidonic acid metabolism:indomethacin, an inhibitor of the cyclooxygenase system; nordihydroguaiaretic acid, an antioxidant that inhibits lipoxygenase; and icosatetraynoic acid, an acetylenic analogue of arachidonic acid that blocks all known pathways of arachidonic acid metabolism. Indomethacin was ineffective in blocking luteinizing hormone-releasing hormone-stimulated luteinizing hormone secretion. Nordihydroguaiaretic acid was only marginally capable of inhibiting luteinizing hormone-releasing hormone-stimulated luteinizing hormone secretion. Icosatetraynoic acid at 10 microM completely inhibited stimulated luteinizing hormone secretion. Addition of several epoxygenated arachidonic acid metabolites to cells in vitro resulted in secretion of luteinizing hormone equal to or greater than that induced by 10 nM luteinizing hormone-releasing hormone. The half-maximal effective dose for these compounds was approximately 50 nM. The 5,6-epoxyicosatrienoic acid was the most potent of the compounds tested. These studies suggest that luteinizing hormone-releasing hormone-stimulated luteinizing hormone release is closely coupled with the production of oxidized arachidonic acid metabolites. Moreover, one or more of the epoxygenated arachidonic acid metabolites might be a component of the cascade of reactions initiated by luteinizing hormone-releasing hormone that ultimately results in secretion of luteinizing hormone.
G D Snyder; J Capdevila; N Chacos; S Manna; J R Falck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  80     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-07-08     Completed Date:  1983-07-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3504-7     Citation Subset:  IM    
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MeSH Terms
5,8,11,14-Eicosatetraynoic Acid / pharmacology
Arachidonic Acids / metabolism,  pharmacology*
Catechols / pharmacology
Gonadotropin-Releasing Hormone / pharmacology*
Indomethacin / pharmacology
Luteinizing Hormone / secretion*
Nordihydroguaiaretic Acid
Pituitary Gland, Anterior / secretion*
Rats, Inbred Strains
Structure-Activity Relationship
Grant Support
Reg. No./Substance:
0/Arachidonic Acids; 0/Catechols; 1191-85-1/5,8,11,14-Eicosatetraynoic Acid; 33515-09-2/Gonadotropin-Releasing Hormone; 500-38-9/Nordihydroguaiaretic Acid; 53-86-1/Indomethacin; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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