| Action of delta 9-tetrahydrocannabinol on cell division and macromolecular synthesis in division-synchronized protozoa. | |
| | |
MedLine Citation:
|
PMID: 819944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Delta 9-Tetrahydrocannabinol (THC) elicited a dose-dependent (3.2-24 muM) response for form/movement, cellular growth and division in log growth phase and division-synchronized Tetrahymena pyriformis GL. Progressive dose-dependent action of THC on division delays in division-synchronized cell cultures was correlated with a concomitant reduction of division maxima and the percent of cells that completed division I. THC depressed the incorporation of 5-3H-uridine, 2-14C-thymidine and L-3-14C-phenylalanine into RNA, DNA and protein macromolecules respectively of division-synchronized Tetrahymena during division I. The depression of incorporation of 5-3H-uridine into nucleic acid macromolecules was correlated with a reduction of exogenous precursor in the cellular pool. The specific activity of radiolabeled mRNA and nascent polypeptides of polyribosomal fractions from synchronized cells was reduced by THC treatment. THC caused an inhibition of the incorporation of 5-3H-uridine into ribosomal RNA (17S and 25S RNA) and ribosomal precursor RNA (35S RNA) of synchronized cells. |
| | |
Authors:
|
D K McClean; A M Zimmerman |
Related Documents
:
|
21546454 - Cell wall damage-induced lignin biosynthesis is regulated by a ros- and jasmonic acid d... 5327364 - Repair of radiation-induced damage to the cell division mechanism of escherichia coli. 19572334 - Mitosis in diatoms: rediscovering an old model for cell division. 19906584 - Cell shape and cell division in fission yeast. 21496344 - Antisense oligodeoxynucleotides targeting atm strengthen apoptosis of laryngeal squamou... 18419814 - Proteome alteration induced by htert transfection of human fibroblast cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Pharmacology Volume: 14 ISSN: 0031-7012 ISO Abbreviation: Pharmacology Publication Date: 1976 |
Date Detail:
|
Created Date: 1976-09-01 Completed Date: 1976-09-01 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0152016 Medline TA: Pharmacology Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 307-21 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cannabis
/
pharmacology* Cell Division / drug effects* Cells, Cultured DNA / biosynthesis* Dose-Response Relationship, Drug Macromolecular Substances Polyribosomes / drug effects, metabolism RNA / biosynthesis* Tetrahydrocannabinol / administration & dosage, pharmacology* Tetrahymena pyriformis / drug effects*, growth & development, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Macromolecular Substances; 1972-08-3/Tetrahydrocannabinol; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Stability of injection solutions of vitamin B1 Part 1: Influence of chelating agents.
Next Document: A Cerenkov detector for energy calibration of electron accelerators in clinical use.