Document Detail


Action of chondroitinases. II. Numerical calculation of the degree of multiple attack.
MedLine Citation:
PMID:  1018015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Further investigation was carried out on the action patterns of two chondroitinase-AC [EC 4.2.2.5.] preparations obtained from Arthrobacter aurescens and Flavobacterium heparinum. To infer the action patterns of the chondroitinases, we proposed a new method for the calculation of the degree of multiple attack, based on the concept established by Robyt and French ((1967) Arch. Biochem. Biophys. 122, 8-16). It was shown that the degree of multiple attack (DM) is represented by the ratio of the initial velocity of number-average degree of scission to that of viscosity-average degree of scission. By this method, DM for A-Chase was estimated to be 3.03 and for F-chase, 1.31.
Authors:
K Hiyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  80     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1976 Dec 
Date Detail:
Created Date:  1977-04-15     Completed Date:  1977-04-15     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1209-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arthrobacter / enzymology
Chondroitin Lyases / metabolism*
Chondroitin Sulfates / metabolism
Chondroitinases and Chondroitin Lyases / metabolism*
Flavobacterium / enzymology
Models, Chemical
Molecular Weight
Viscosity
Chemical
Reg. No./Substance:
9007-28-7/Chondroitin Sulfates; EC 4.2.2.-/Chondroitin Lyases; EC 4.2.2.-/Chondroitinases and Chondroitin Lyases

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