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Action of Bacillus subtilis alpha-amylase on native wheat starch.
MedLine Citation:
PMID:  18584697     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Native starch granules from wheat have been subjected to enzymatic depolymerization with an alpha-amylase from Bacillus subtilis. Crystallites made from short-chain amylose and residues from mild acid hydrolysis have been also tested. Electron microscopy, particle size analysis, DSC, and x-ray diffractometry reveal that enzymatic degradation occurs granule by granule. Gel permeation chromatography shows off the macromolecular nature of the remaining material. In contrast, acid erodes simultaneously all the granules, leading to a splitting into small particles. Crystalline fractions are completely degraded by alpha-amylase. These results support evidence for an active disentanglement of chains, carried out by the different subsites of alpha-amylase molecules. A simple mathematical treatment is proposed to explain the results of the kinetics.
Authors:
P Colonna; A Buléon; F Lemarié
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  31     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  2008-06-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  895-904     Citation Subset:  -    
Affiliation:
Institut National de la Recherche Agronomique, Centre de Recherches Agro-Alimentaires, Rue de la Géraudière, 44072 Nantes Cédex 03, France.
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