Document Detail


Actinomycin D: effects on mouse L-cells.
MedLine Citation:
PMID:  5357735     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lethal and inhibitory effects of actinomycin D (Act D) on asynchronous and synchronized populations of mouse L-cells have been studied. It has been shown that the survival curve of populations in the logarithmic phase of growth can be approximated by two exponential survival curves corresponding to a sensitive and resistant moiety. The size and sensitivity of both moieties vary during the growth of the population. As the cell population moves through logarithmic and into stationary phase, the sensitive moiety becomes smaller but more resistant whereas the resistant moiety increases in size and also becomes more resistant. This variation appears to be related to a reduced uptake of Act D and also a reduced rate of DNA and RNA synthesis. Variations in sensitivity to the drug have also been observed during the division cycle of synchronized cells with cells in the S phase showing the greatest uptake of the drug and also the greatest sensitivity. However, no direct correlation between uptake and sensitivity has been established. Actinomycin D has inhibitory effects on both RNA and DNA synthesis. RNA synthesis is inhibited rapidly but does not seem to drop to less than 5% of the control value. The inhibition of DNA synthesis appears to occur over a longer period and may reach values as low as 0.25% of control. In both cases the degree of inhibitions appears to be dependent on both the length of exposure and the concentration of the drug. Certain similarities between the response of cells to Act D and X-rays have been observed and are discussed.
Authors:
S Bacchetti; G F Whitmore
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biophysical journal     Volume:  9     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1969 Dec 
Date Detail:
Created Date:  1970-01-28     Completed Date:  1970-01-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1427-45     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects*
DNA / biosynthesis
Dactinomycin / pharmacology*
L Cells (Cell Line)*
Mice
RNA / biosynthesis
Time
Tritium
Uridine / metabolism
Chemical
Reg. No./Substance:
10028-17-8/Tritium; 50-76-0/Dactinomycin; 58-96-8/Uridine; 63231-63-0/RNA; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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