Document Detail

Actin filaments and microtubules regulate endocytosis in marginal cells of the stria vascularis.
MedLine Citation:
PMID:  18608006     Owner:  NLM     Status:  MEDLINE    
CONCLUSION: Cationized ferritin (CF) was internalized via clathrin-mediated endocytosis. This process depends on clathrin, actin filaments, and microtubules. Microperoxidase (MPO) was internalized via a clathrin- and caveolin-independent endocytic pathway, which was partially dependent on microtubules but independent of clathrin and actin filaments. OBJECTIVE: We investigated the role of actin filaments and microtubules in the transport of endocytic carrier vesicles (ECVs) from the plasma membrane to the early sorting endosomes, using CF and MPO as tracers. MATERIALS AND METHODS: Fifty-five guinea pigs were used. The animals were divided into a CF endocytosis group and an MPO endocytosis group. These groups consisted of control, nocodazole-treated, cytochalasin (Cyt D)-treated, Cyt D + nocodazole-treated, and geldanamycin-treated subgroups. RESULTS: For CF endocytosis, the following results were obtained. In the nocodazole experiment, in which microtubules were disrupted to form monomeric tubulin, the number of ECVs loaded with CF was greatly decreased. In the Cyt D experiment, in which the actin filaments were disrupted to form monomers, the number of ECVs labeled with CF was also greatly decreased. In the geldanamycin experiment, in which clathrin-mediated endocytosis was regulated and actin stress fibers were dissolved, the endocytosis of CF was severely inhibited. For MPO endocytosis, in the nocodazole experiment, the endocytosis of MPO was markedly suppressed.
Akinobu Kakigi; Teruhiko Okada; Taizo Takeda; Daizo Taguchi; Rie Nishioka; Masahiko Nishimura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  128     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-09-09     Completed Date:  2009-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  856-60     Citation Subset:  IM    
Department of Otolaryngology, Kochi Medical School, Kochi, Japan.
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MeSH Terms
Biological Transport / physiology
Cell Membrane / metabolism
Endocytosis / physiology*
Endosomes / metabolism
Ferritins / pharmacokinetics
Guinea Pigs
Microfilaments / physiology*
Microtubules / physiology*
Peroxidases / pharmacokinetics
Stria Vascularis / cytology*,  physiology
Reg. No./Substance:
0/polycationic ferritin; 9007-73-2/Ferritins; EC 1.11.1.-/Peroxidases; EC 1.11.1.-/microperoxidase

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