Document Detail


Actin filament elasticity and retrograde flow shape the force-velocity relation of motile cells.
MedLine Citation:
PMID:  22339865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells migrate through a crowded environment during processes such as metastasis or wound healing, and must generate and withstand substantial forces. The cellular motility responses to environmental forces are represented by their force-velocity relation, which has been measured for fish keratocytes but remains unexplained. Even pN opposing forces slow down lamellipodium motion by three orders of magnitude. At larger opposing forces, the retrograde flow of the actin network accelerates until it compensates for polymerization, and cell motion stalls. Subsequently, the lamellipodium adapts to the stalled state. We present a mechanism quantitatively explaining the cell's force-velocity relation and its changes upon application of drugs that hinder actin polymerization or actomyosin-based contractility. Elastic properties of filaments, close to the lamellipodium leading edge, and retrograde flow shape the force-velocity relation. To our knowledge, our results shed new light on how these migratory responses are regulated, and on the mechanics and structure of the lamellipodium.
Authors:
Juliane Zimmermann; Claudia Brunner; Mihaela Enculescu; Michael Goegler; Allen Ehrlicher; Josef Käs; Martin Falcke
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biophysical journal     Volume:  102     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-06-07     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  287-95     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Mathematical Cell Physiology, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany. juliane.zimmermann@mdc-berlin.de
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / drug effects,  metabolism*
Animals
Azepines / pharmacology
Biomechanics
Cell Movement* / drug effects
Corneal Keratocytes / cytology
Cytochalasin D / pharmacology
Elasticity* / drug effects
Goldfish
Microscopy, Atomic Force
Models, Biological*
Naphthalenes / pharmacology
Chemical
Reg. No./Substance:
0/Azepines; 0/Naphthalenes; 109376-83-2/ML 7; 22144-77-0/Cytochalasin D
Comments/Corrections

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