Document Detail


Actin-dependent cytoplasmic streaming in C. elegans oogenesis.
MedLine Citation:
PMID:  17507392     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oocytes in the C. elegans gonad enlarge rapidly. During the stage of enlargement, they are transcriptionally quiescent, and it is not understood how they acquire large quantities of materials such as mRNA and protein. Enlarging oocytes are connected via cytoplasmic bridges to a large, younger population of transcriptionally active germ cells at various stages of mitosis and meiosis. We show here that there is a general streaming of gonad cytoplasm towards and into the enlarging oocytes, originating primarily from pachytene-stage germ cells. Because previous studies suggested that most or all of the pachytene germ cells have the potential to differentiate into oocytes, the pachytene cells appear to function transiently as nurse cells. Somatic gonadal cells that surround the germ cells do not appear essential for streaming. Instead, materials appear to be pulled into oocytes by forces generated either in, or adjacent to, the enlarging oocytes themselves. Streaming appears to be driven by the actomyosin cytoskeleton, although we show that populations of both microfilaments and microtubules are oriented in the direction of flow. Our study shows that oocyte enlargement in C. elegans differs significantly from that in Drosophila, where a small number of specialized nurse cells expel their contents into the enlarging oocyte.
Authors:
Uta Wolke; Erin A Jezuit; James R Priess
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-16
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  134     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-31     Completed Date:  2007-07-30     Revised Date:  2014-02-21    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2227-36     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Animals, Genetically Modified
Caenorhabditis elegans / cytology*,  genetics,  physiology*
Cytoplasmic Streaming / physiology*
Female
Models, Biological
Oocytes* / cytology,  physiology
Oogenesis / physiology*
RNA Interference
Grant Support
ID/Acronym/Agency:
T32 HD007183/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Actins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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