Document Detail


Actin cytoskeleton architecture and signaling in osmosensing.
MedLine Citation:
PMID:  17875420     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since the early days of cell volume regulation research, the role of actin cytoskeleton organization and rearrangement has attracted specific interest. Rapid modifications in actin dynamics and architecture have been described. They were shown to regulate cell volume changes, as well as regulatory volume decrease in a large variety of cell types, including hepatocytes, lymphocytes, fibroblasts, myocytes, and various tumor cells. Using microscopic and biochemical analyses, modifications of actin organization and polymerization dynamics were studied. This chapter summarizes the molecular approaches applied so far for the quantitative assessment of actin cytoskeleton dynamics in the various cell types. It demonstrates that rapid modifications of actin cytoskeleton dynamics regulated by specific signaling pathways play a functional role in cell volume regulation. It is concluded that studying actin polymerization dynamics and signaling represents a challenging tool for the understanding of osmosensing and osmosignaling regulation in cellular physiology.
Authors:
Evangelia A Papakonstanti; Christos Stournaras
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  428     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-09-18     Completed Date:  2007-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  227-40     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Crete Medical School, Heraklion, Greece.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / ultrastructure*
Animals
Cell Size*
Cytoskeleton / ultrastructure*
Deoxyribonuclease I / antagonists & inhibitors
Humans
Immunoblotting / methods
Octoxynol
Osmotic Pressure*
Phalloidine
Phenylmethylsulfonyl Fluoride / pharmacology
Rhodamines
Signal Transduction / physiology*
Solubility
Chemical
Reg. No./Substance:
0/Actins; 0/Rhodamines; 17466-45-4/Phalloidine; 329-98-6/Phenylmethylsulfonyl Fluoride; 9002-93-1/Octoxynol; EC 3.1.21.1/Deoxyribonuclease I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cell volume regulatory ion channels in cell proliferation and cell death.
Next Document:  Osmotic stress and DNA damage.