Document Detail

Actin cytoskeleton architecture and signaling in osmosensing.
MedLine Citation:
PMID:  17875420     Owner:  NLM     Status:  MEDLINE    
Since the early days of cell volume regulation research, the role of actin cytoskeleton organization and rearrangement has attracted specific interest. Rapid modifications in actin dynamics and architecture have been described. They were shown to regulate cell volume changes, as well as regulatory volume decrease in a large variety of cell types, including hepatocytes, lymphocytes, fibroblasts, myocytes, and various tumor cells. Using microscopic and biochemical analyses, modifications of actin organization and polymerization dynamics were studied. This chapter summarizes the molecular approaches applied so far for the quantitative assessment of actin cytoskeleton dynamics in the various cell types. It demonstrates that rapid modifications of actin cytoskeleton dynamics regulated by specific signaling pathways play a functional role in cell volume regulation. It is concluded that studying actin polymerization dynamics and signaling represents a challenging tool for the understanding of osmosensing and osmosignaling regulation in cellular physiology.
Evangelia A Papakonstanti; Christos Stournaras
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  428     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-09-18     Completed Date:  2007-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  227-40     Citation Subset:  IM    
Department of Biochemistry, University of Crete Medical School, Heraklion, Greece.
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MeSH Terms
Actins / ultrastructure*
Cell Size*
Cytoskeleton / ultrastructure*
Deoxyribonuclease I / antagonists & inhibitors
Immunoblotting / methods
Osmotic Pressure*
Phenylmethylsulfonyl Fluoride / pharmacology
Signal Transduction / physiology*
Reg. No./Substance:
0/Actins; 0/Rhodamines; 17466-45-4/Phalloidine; 329-98-6/Phenylmethylsulfonyl Fluoride; 9002-93-1/Octoxynol; EC I

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