Document Detail

Actin microfilament involved in regulation of pacemaking activity in cultured interstitial cells of Cajal from murine intestine.
MedLine Citation:
PMID:  20349180     Owner:  NLM     Status:  MEDLINE    
The present study investigated the effect of actin microfilament structure on pacemaker currents and calcium oscillation in cultured murine intestinal interstitial cells of Cajal (ICCs) by whole-cell patch-clamp technique and calcium imaging technique. Cytochalasin B, a disruptor of actin microfilaments, decreased the amplitude and frequency of pacemaker currents from 491.32 +/- 160.33 pA and 11.73 +/- 0.79 cycles/min to 233.12 +/- 92.00 pA and 10.29 +/- 0.76 cycles/min. Cytochalasin B also decreased the amplitude and frequency of calcium oscillation from 0.32 +/- 0.08 (DeltaF/F0) and 2.75 +/- 0.17 cycles/min to 0.02 +/- 0.01 (DeltaF/F0) and 1.20 +/- 0.08 cycles/min. Phalloidin, a stabilizer of actin microfilaments, increased the amplitude and frequency of pacemaker currents from 751.79 +/- 282.82 pA and 13.93 +/- 1.00 cycles/min to 1234.34 +/- 607.83 pA and 14.68 +/- 1.00 cycles/min. Phalloidin also increased the amplitude and frequency of calcium oscillation from 0.26 +/- 0.01 (DeltaF/F0) and 2.27 +/- 0.18 cycles/min to 0.43 +/- 0.03 (DeltaF/F0) and 2.87 +/- 0.07 cycles/min. 2-Aminoethoxydiphenyl borane (2-APB), an IP(3) receptor blocker, suppressed both pacemaker currents and calcium oscillations. 2-APB also blocked the phalloidin-induced increase in pacemaker currents and calcium oscillation. Ryanodine, an inhibitor of calcium-induced calcium release, did not affect pacemaker current but suppressed calcium oscillations. Ryanodine had no effect on altering phalloidin-induced increases in pacemaker current and calcium oscillation. These results suggest that actin microfilaments regulate pacemaker activity via the IP(3)-induced calcium release signaling pathway.
Zuo Yu Wang; Yan Fei Han; Xu Huang; Hong Li Lu; Xin Guo; Yong Chul Kim; Wen Xie Xu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-27
Journal Detail:
Title:  The Journal of membrane biology     Volume:  234     ISSN:  1432-1424     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-12     Completed Date:  2010-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  217-25     Citation Subset:  IM    
Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, 200240, China.
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MeSH Terms
Actins / metabolism
Biological Clocks / drug effects
Calcium / metabolism
Cytochalasin B / pharmacology
Interstitial Cells of Cajal / drug effects,  metabolism*
Mice, Inbred BALB C
Microfilaments / metabolism
Patch-Clamp Techniques
Phalloidine / pharmacology
Ryanodine / pharmacology
Ryanodine Receptor Calcium Release Channel / metabolism
Reg. No./Substance:
0/Actins; 0/Ryanodine Receptor Calcium Release Channel; 14930-96-2/Cytochalasin B; 15662-33-6/Ryanodine; 17466-45-4/Phalloidine; 7440-70-2/Calcium

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