| Acrylonitrile induced apoptosis via oxidative stress in neuroblastoma SH-SY5Y cell. | |
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MedLine Citation:
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PMID: 20981856 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Acrylonitrile (ACN) is a chemical that is widely used in the production of plastics, acrylic fibers, synthetic rubbers and resins. It has been reported that ACN can cause oxidative stress, a condition which is well recognized as an apoptotic initiator; however, information regarding ACN-induced apoptosis is limited. This present study investigated whether ACN induces apoptosis in human neuroblastoma SH-SY5Y cells, and whether its apoptotic induction involves oxidative stress. The results showed that ACN caused activation of caspase-3, a key enzyme involved in apoptosis, in a dose- and time-dependent manner. Detection of sub-G1 apoptotic cell death and apoptotic nuclear condensation revealed that ACN caused an increase in the number of apoptotic cells indicating ACN induces apoptosis in SH-SY5Y cells. ACN dose- and time-dependently increased the level of proapoptotic protein, Bax. Pretreatment with N-acetylcysteine (NAC), an antioxidant, attenuated caspase-3 activation by ACN, as evidenced by a reduction in proteolysis of PARP, a known caspase-3 substrate, as well as in the number of sub-G1 apoptotic cells. Moreover, induction of Bax by ACN was abolished by NAC. Taken together, the results indicate that ACN induces apoptosis in SH-SY5Y cells via a mechanism involving generation of oxidative stress-mediated Bax induction. |
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Authors:
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Piyajit Watcharasit; Sumitra Suntararuks; Daranee Visitnonthachai; Apinya Thiantanawat; Jutamaad Satayavivad |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied toxicology : JAT Volume: 30 ISSN: 1099-1263 ISO Abbreviation: J Appl Toxicol Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8109495 Medline TA: J Appl Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 649-55 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
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Laboratory of Pharmacology, Chulabhorn Research Institute, 54 Moo 4 Vibhavadee-Rangsit Highway, Bangkok 10210, Thailand. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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