Document Detail

Acrosomal ultrastructure of stallion spermatozoa cryopreserved with ethylene glycol using two packaging systems.
MedLine Citation:
PMID:  11093629     Owner:  NLM     Status:  MEDLINE    
The present experiments aimed to examine the substitution of glycerol (G) by ethylene glycol (E) as a cryoprotective agent for stallion spermatozoa. Two different ethylene glycol concentrations (5% and 10%) and also the association of glycerol (2%) and ethylene glycol (3%) (E/G) were studied (Experiment 1). In Experiment 2, two packing systems (0.5 x 4.0 ml) were evaluated using both cryoprotectors. In both experiments, the sperm membrane integrity after freezing was evaluated using transmission electron microscopy. The mean post-thaw motility was 34.25, 36.5, 29.25 and 34.75% for G5%, E5%, E10% and E/G, respectively. It was observed that the percentage of motile spermatozoa was significantly smaller (P<0.05) when semen was processed with E10%. A decrease in the acrosome integrity was observed in frozen thawed spermatozoa from all treated groups. It was observed that 28.0, 22.5, 25.5 and 22.5% of the sperm cells had a normal acrosome following freezing with G5%, E5%, E10% and E/G, respectively. Undulation of the outer acrosomal membrane, acrosomal swelling and loss of acrosomal content density and homogeneity were the most evident ultrastructural alterations observed. In Experiment 2, the post-thaw motility was higher (P<0.05) for sperm frozen in 0.5 ml straws than in 4.0 ml straws, regardless of the cryoprotector used. The ultrastructural evaluation showed 26.7 and 16.0% of intact acrosomes for sperm frozen in 0.5 ml and 4.0 ml straws, respectively. We concluded that ethylene glycol has similar cryoprotective properties to glycerol and that utilisation of 0.5 ml straws improved the ability of horse sperm cells to withstand damage after the cryopreservation process.
M A Alvarenga; F C Landim-Alvarenga; R M Moreira; M M Cesarino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Equine veterinary journal     Volume:  32     ISSN:  0425-1644     ISO Abbreviation:  Equine Vet. J.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-01-02     Completed Date:  2001-01-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0173320     Medline TA:  Equine Vet J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  541-5     Citation Subset:  IM    
Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu SP, Brazil.
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MeSH Terms
Acrosome / ultrastructure*
Cryopreservation / veterinary
Ethylene Glycol
Horses / anatomy & histology*
Semen Preservation / methods,  veterinary*
Spermatozoa / ultrastructure*
Reg. No./Substance:
107-21-1/Ethylene Glycol; 56-81-5/Glycerol

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