| Acroplaxome, an F-actin-keratin-containing plate, anchors the acrosome to the nucleus during shaping of the spermatid head. | |
| | |
MedLine Citation:
|
PMID: 14551252 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Nuclear shaping is a critical event during sperm development as demonstrated by the incidence of male infertility associated with abnormal sperm ad shaping. Herein, we demonstrate that mouse and rat spermatids assemble in the subacrosomal space a cytoskeletal scaffold containing F-actin and Sak57, a keratin ortholog. The cytoskeletal plate, designated acroplaxome, anchors the developing acrosome to the nuclear envelope. The acroplaxome consists of a marginal ring containing keratin 5 10-nm-thick filaments and F-actin. The ring is closely associated with the leading edge of the acrosome and to the nuclear envelope during the elongation of the spermatid head. Anchorage of the acroplaxome to the gradually shaping nucleus is not disrupted by hypotonic treatment and brief Triton X-100 extraction. By examining spermiogenesis in the azh mutant mouse, characterized by abnormal spermatid/sperm head shaping, we have determined that a deformity of the spermatid nucleus is restricted to the acroplaxome region. These findings lead to the suggestion that the acroplaxome nucleates an F-actin-keratin-containing assembly with the purpose of stabilizing and anchoring the developing acrosome during spermatid nuclear elongation. The acroplaxome may also provide a mechanical planar scaffold modulating external clutching forces generated by a stack of Sertoli cell F-actin-containing hoops encircling the elongating spermatid nucleus. |
| | |
Authors:
|
Abraham L Kierszenbaum; Eugene Rivkin; Laura L Tres |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2003-08-07 |
Journal Detail:
|
Title: Molecular biology of the cell Volume: 14 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2003 Nov |
Date Detail:
|
Created Date: 2003-10-31 Completed Date: 2004-04-02 Revised Date: 2013-04-18 |
Medline Journal Info:
|
Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
|
Languages: eng Pagination: 4628-40 Citation Subset: IM |
Affiliation:
|
Department of Cell Biology and Anatomical Sciences, The Sophie Davis School of Biomedical Education/The City University of New York Medical School, New York New York 10031, USA. kier@med.cuny.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acrosome
/
metabolism*,
ultrastructure Actins / metabolism* Amino Acid Sequence Animals Cell Nucleus / metabolism*, ultrastructure Cloning, Molecular Fluorescent Antibody Technique Gene Library Humans Keratin-5 Keratins / metabolism* Male Mice Microscopy, Immunoelectron Molecular Sequence Data Nuclear Envelope / metabolism, ultrastructure Rats Sequence Analysis, Protein Skin / cytology, metabolism Spermatids / metabolism*, ultrastructure Spermatogenesis / physiology Testis / cytology, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
HD37282/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Actins; 0/KRT5 protein, human; 0/Keratin-5; 0/Krt1-5 protein, mouse; 0/Krt2-5 protein, rat; 68238-35-7/Keratins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Targeted chemical disruption of clathrin function in living cells.
Next Document: PIKfyve controls fluid phase endocytosis but not recycling/degradation of endocytosed receptors or s...