Document Detail


Acroplaxome, an F-actin-keratin-containing plate, anchors the acrosome to the nucleus during shaping of the spermatid head.
MedLine Citation:
PMID:  14551252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nuclear shaping is a critical event during sperm development as demonstrated by the incidence of male infertility associated with abnormal sperm ad shaping. Herein, we demonstrate that mouse and rat spermatids assemble in the subacrosomal space a cytoskeletal scaffold containing F-actin and Sak57, a keratin ortholog. The cytoskeletal plate, designated acroplaxome, anchors the developing acrosome to the nuclear envelope. The acroplaxome consists of a marginal ring containing keratin 5 10-nm-thick filaments and F-actin. The ring is closely associated with the leading edge of the acrosome and to the nuclear envelope during the elongation of the spermatid head. Anchorage of the acroplaxome to the gradually shaping nucleus is not disrupted by hypotonic treatment and brief Triton X-100 extraction. By examining spermiogenesis in the azh mutant mouse, characterized by abnormal spermatid/sperm head shaping, we have determined that a deformity of the spermatid nucleus is restricted to the acroplaxome region. These findings lead to the suggestion that the acroplaxome nucleates an F-actin-keratin-containing assembly with the purpose of stabilizing and anchoring the developing acrosome during spermatid nuclear elongation. The acroplaxome may also provide a mechanical planar scaffold modulating external clutching forces generated by a stack of Sertoli cell F-actin-containing hoops encircling the elongating spermatid nucleus.
Authors:
Abraham L Kierszenbaum; Eugene Rivkin; Laura L Tres
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2003-08-07
Journal Detail:
Title:  Molecular biology of the cell     Volume:  14     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-31     Completed Date:  2004-04-02     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4628-40     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Anatomical Sciences, The Sophie Davis School of Biomedical Education/The City University of New York Medical School, New York New York 10031, USA. kier@med.cuny.edu
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MeSH Terms
Descriptor/Qualifier:
Acrosome / metabolism*,  ultrastructure
Actins / metabolism*
Amino Acid Sequence
Animals
Cell Nucleus / metabolism*,  ultrastructure
Cloning, Molecular
Fluorescent Antibody Technique
Gene Library
Humans
Keratin-5
Keratins / metabolism*
Male
Mice
Microscopy, Immunoelectron
Molecular Sequence Data
Nuclear Envelope / metabolism,  ultrastructure
Rats
Sequence Analysis, Protein
Skin / cytology,  metabolism
Spermatids / metabolism*,  ultrastructure
Spermatogenesis / physiology
Testis / cytology,  metabolism
Grant Support
ID/Acronym/Agency:
HD37282/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/KRT5 protein, human; 0/Keratin-5; 0/Krt1-5 protein, mouse; 0/Krt2-5 protein, rat; 68238-35-7/Keratins
Comments/Corrections

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