Document Detail

Acquisition of competence to condense metaphase I chromosomes during spermatogenesis.
MedLine Citation:
PMID:  9882497     Owner:  NLM     Status:  MEDLINE    
Little is known about the timing of meiotic prophase events during spermatogenesis in the mouse or how these events are related to cell-cycle progression. This work was designed to test hypotheses about the timing and biochemical correlates of developmental acquisition of competence to condense bivalent pairs of homologous chromosomes held together by chiasmata. The experimental approach takes advantage of the fact that okadaic acid (OA) treatment of pachytene spermatocytes causes precocious entry into metaphase I (MI) of meiosis. Leptotene and zygotene (L/Z) spermatocytes are not competent to respond to OA with condensation of chiasmate bivalent chromosomes. Competence for MI condensation of chiasmate bivalents is acquired by the middle of the pachytene stage of meiotic prophase, several days after homologous chromosomes become fully synapsed. The acquisition of MI competence is paralleled by the accumulation of histone H1t in the nuclei of mid-pachytene spermatocytes. Biochemical differences also exist between the incompetent L/Z spermatocytes and the competent pachytene spermatocytes. Both have the molecular components of metaphase promoting factor, CDC2 and CYCLIN B1; however, the histone H1 kinase activity of metaphase promoting factor of incompetent L/Z spermatocytes is not activated by OA, as it is in competent pachytene spermatocytes. Additionally, the CDC25C protein phosphatase is present in competent pachytene spermatocytes, but not in incompetent L/Z or early pachytene spermatocytes. Both incompetent and competent spermatocytes accumulate MPM-2 phosphoepitopes and phosphorylated histone H3 in response to OA treatment, indicating that presence of these antigens is not sufficient to promote condensation of meiotic chromosomes. These data demonstrate that meiotic competence of spermatocytes is acquired after homologous chromosome pairing is established and is coincident with first appearance of histone H1t and CDC25C protein phosphatase in spermatocytes.
J Cobb; B Cargile; M A Handel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  205     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-05     Completed Date:  1999-02-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  49-64     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee, 37996, USA.
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MeSH Terms
CDC2 Protein Kinase / metabolism
Cell Cycle / physiology*
Cell Cycle Proteins / metabolism
Cell Nucleus / physiology
Chromatin / physiology
Chromosomes / drug effects,  genetics,  physiology*
Cyclin B / metabolism
Cyclin B1
Histones / metabolism
Mice, Inbred ICR
Okadaic Acid / pharmacology
Protein Kinases / metabolism
Protein-Serine-Threonine Kinases / metabolism
Spermatocytes / cytology,  physiology*
Spermatogenesis / genetics*
cdc25 Phosphatases*
Grant Support
Reg. No./Substance:
0/Ccnb1 protein, mouse; 0/Cell Cycle Proteins; 0/Chromatin; 0/Cyclin B; 0/Cyclin B1; 0/Histones; 78111-17-8/Okadaic Acid; EC 2.7.-/Protein Kinases; EC 2.7.1.-/histone H1 kinase; EC Kinases; EC Protein Kinase; EC protein, mouse; EC Phosphatases

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